Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 218-301-2 | CAS number: 2110-78-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Daphnia sp. Acute Immobilization Test (OECD TG 202): 24- and 48-hour EC50 >100 mg/L
Alga Growth Inhibition Test (OECD TG 201): Cell yield 72-hour EyC50 54.8 mg/L, growth rate 72-hour ErC50 >100 mg/L, growth rate 72-hour ErC20 95.6 mg/L
Additional information
Toxicity to aquatic invertebrates
A Daphnia sp. Acute Immobilization Test in accordance with OECD Testing Guideline 202 and GLP principles was designed to assess the potential effects of methyl 2-hydroxyisobutyrate to the freshwater cladoceran, Daphnia magna. The study was conducted as a limit test with four replicate vessels of five daphnids per test level exposed to nominal test concentrations of 0 (water control) and 100 mg methyl 2-hydroxyisobutyrate/L, over a 48-hour static exposure period. Observations were made at 24 and 48 hours (±1 hour from test initiation) for daphnid immobility (inability to swim within approximately 15 seconds after gentle agitation of the test vessel) and any changes in behavior or appearance. Temperatures during the exposure period ranged from 20-21°C. Dissolved oxygen levels ranged from 8.6-8.8 mg/L (97-101% air saturation). The pH ranged from 7.7-7.8 and the light intensity ranged from 354-414 lux. Test solutions were analyzed for methyl 2-hydroxyisobutyrate concentrations at test initiation and exposure termination by gas chromatography with selected ion monitoring (GC/SIM).
None of the analyses of the water control exhibited a concentration exceeding the lower limit of quantitation (LLQ) equivalent to 0.125 mg methyl 2-hydroxyisobutyrate/L. Measured concentrations of methyl 2-hydroxyisobutyrate at the limit test level ranged from 82.1 to 92.6% of the nominal concentration over the course of the exposure period. The resulting mean measured concentration was 87.4 mg methyl 2-hydroxyisobutyrate/L, equating to 87.4% of the nominal concentration. Because the mean measured concentration was within ± 20% of the nominal target concentration, results were based on the nominal (limit) concentration.
The acute toxicity values for daphnid (D. magna) exposed to methyl 2-hydroxyisobutyrate over a 48-hour exposure period and based on nominal concentrations were as follows: 24- and 48-hour EC50 : >100 mg/L (limit concentration tested); 48-hour NOEC: 100 mg/L (limit concentration tested)
Toxicity to algae
To assess potential effects of methyl 2-hydroxyisobutyrate to the freshwater green alga, Pseudokirchneriella subcapitata, an Alga Growth Inhibition Test according to OECD Testing Guideline 201 was performed for 72 hours with target concentrations of 0 (AAP control), 1.00, 3.10, 9.80, 31.3 and 100 mg methyl 2-hydroxyisobutyrate/L. Cell density was determined at approximately 24, 48 and 72 hours (±1 hour from test initiation). Temperatures during the exposure period ranged from 22-23°C. The pH ranged from 7.3-7.8 and the light intensity ranged from 4680-5520 lux. Test solutions were analyzed for methyl 2-hydroxyisobutyrate concentrations at test initiation and exposure termination by gas chromatography with selected ion monitoring (GC/SIM). None of the analyses of the AAP medium control exhibited a concentration exceeding the lower limit of quantitation (LLQ) equivalent to 0.125 mg methyl 2-hydroxyisobutyrate/L. Measured concentrations of methyl 2-hydroxyisobutyrate ranged from 83.2 to 104% of nominal concentrations over the course of the exposure period. The resulting mean measured test concentrations were 0.959, 2.99, 8.92, 28.7 and 91.2 mg methyl 2-hydroxyisobutyrate/L. Because the mean measured concentration were within ± 20% of the target nominal concentrations, study endpoints were based on nominal concentrations.
The acute toxicity values for Pseudokirchneriella subcapitata exposed to methyl 2-hydroxyisobutyrate over a 72-hour static exposure period and based on nominal concentrations were as follows:
Growth rate
72-hour ErC50: >100 mg/L (highest concentration tested)
72-hour ErC20: 95.6 mg/L (95% C.I. = 84.1 – 109 mg/L)
72-hour ErC10: 45.4 mg/L (95% C.I. = 36.1 – 57.1 mg/L)
72 -hour NOEC: 9.80 mg/L
Cell yield
72-hour EyC50: 54.8 mg/L (95% C.I. = 44.1 – 68.2 mg/L)
72-hour EyC20: 20.8 mg/L (95% C.I. = 13.5 – 32.1 mg/L)
72-hour EyC10: 12.5 mg/L (95% C.I. = 7.03 – 22.3 mg/L)
72-hour NOEC: 9.80 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.