Butane-1,2-diol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38 412, Part 15, Determination of the Effect of Substances in Water on Fish (L15) (Fish-T est). German Institute for Standardization, Berlin. Version June 1982

Version / remarks:

June 1982

Deviations:

no

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Butane-1,2-diol

EC Number:

209-527-2

EC Name:

Butane-1,2-diol

Cas Number:

584-03-2

Molecular formula:

C4H10O2

IUPAC Name:

butane-1,2-diol

Details on test material:

- Name of test material (as cited in study report): 1,2-butanediol
- Physical state: liquid
- Test substance No.: 88/680
- Purtity: 99%
- Water solubility: miscible at any ratio

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

Not applicable

Test solutions

Vehicle:

no

Details on test solutions:

The product was added to the test water without any pretreatment; subsequently the fish were placed in the aquaria.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: golden orfe
- Consigment dated: Oct. 18, 1988
- Supplier/Breeder: Fischzucht Paul Eggers, D-2354 Hohenwestedt, Frg.
- Body length: 7.5 cm (range 6.9-7.9)
- Body weight: 3.6 g (range 2.8-4.6)
- Corpulence factor: 0.9

HOUSING:
- Keeping: Fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air.
- Duration: about 4 months
- Temperature: 20 ± 1 °C
- Photoperiod: 16:8 day:night
- Total hardness: ca. 2.5 mmol/L
- Acid capacity: ca. 5.5 mmol/L
- Oxygen content: >60% of max. saturation
- pH: ca. 8
- Mortality during last week of housing: ca. 0.6%
- Mortality during adaptation period: 0%
- Medical treatment: 4 times with 0.05 mg/L malachite green chloride, 2 times with 10 mg/L tetracycli ne hydrochloride
- Diet: growing feed F/B 50 (SSNIFF Spezialdiaeten GMBH, D-4770 Soest, Frg); ad libitum

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/L

Test temperature:

21 °C

pH:

7.3 - 7.9

Dissolved oxygen:

6.8 - 8.6 mg/L

Nominal and measured concentrations:

- Nominal concentrations: 0 (control), 5000 and 10000 mg/L (based on range-finding test).
- Measured concentrations: no analysis performed.

Details on test conditions:

STUDY DESIGN:
- Test vessel: All-glass aquarium (30x22x24 cm)
- Test volme: 10 L
- Number of animals per test conc.: 10
- Loading (g fish/L): 3.6
- Adaptation period: 3 days
- Photoperiod: 16:8 day:night
- Aeration: slight
- Withdrawal of feed: 1 day before the test.

TEST MEDIUM:
Reconstituted freshwater according to guideline. Preparation from fully demineralised tap water. Conductivity max. micro MHO

EFFECT PARAMETERS MEASURED:
Mortality and general symptoms of toxicity at 1, 4, 24, 48, 72 and 96 hours after the start of the test.

Reference substance (positive control):

yes

Remarks:

chloracetamide

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Mortality: At the 10000 mg/L test group 2/10 deaths were recorded, 24 hours after the start of the test.
Symptoms: No symptoms of toxicity observed.

Results with reference substance (positive control):

48-h LC50: ca. 32 mg/L (determined Feb. 20, 1989)

Reported statistics and error estimates:

Finney, D.J., Probit Analysis, Cambr. Univ. Press, 3rd ed.., 1971 (modified).

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified