Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The test water used for the range-finding and definitive tests was deionized reverse osmosis water containing less than 1 mg/L Dissolved Organic Carbon (DOC).
Synthetic Sewage
A synthetic sewage of the following composition, was added to each test vessel to act as a respiratory substrate:
16 g Peptone, 11 g Meat extract, 3 g Urea, 0.7 g NaCl, 0.4 g CaCl2.2H2O, 0.2 g MgSO4.7H2O, 2.8 g K2HPO4 dissolved in a final volume of 1 liter of water with the aid of ultrasonication.
In the range-finding test, the pH of the synthetic sewage stock was pH 7.3. In the definitive test, the pH of the synthetic sewage stock used to feed the activated sewage sludge was pH 7.4 and the pH of the synthetic sewage stock used for the definitive test was pH 7.2. The pH values were measured using a Hach HQ40d Flexi handheld meter.
Test organisms (species):
activated sludge
Details on inoculum:
A mixed population of activated sewage sludge micro-organisms was obtained on 10 May 2016 for the range-finding test and on 06 July 2016 for the definitive test from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK which treats predominantly domestic sewage.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
21-22 C
pH:
7-8
Dissolved oxygen:
60 - 70 % saturation
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
29 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of heterotrophic respiration
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
66 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of heterotrophic respiration
Results with reference substance (positive control):
EC50 determined in the positive control study with 3,5-dichlorophenol was 6.5 mg/l.
Validity criteria fulfilled:
yes
Conclusions:
The effect of the 1,2-Ethanediamine, N-(2-aminoethyl)-, reaction products with glycidyl tolyl ether on the respiration of activated sewage sludge micro-organisms gave a 3-Hour EC10 value of 29 mg/L and an 3-Hour EC50 value of 66 mg/L.
Executive summary:

The effect of the 1,2-Ethanediamine, N-(2-aminoethyl)-, reaction products with glycidyl tolyl ether on the respiration of activated sewage sludge micro-organisms gave a 3-Hour EC10 value of 29 mg/L and an 3-Hour EC50 value of 66 mg/L.

Description of key information

The effect of the 1,2-Ethanediamine, N-(2-aminoethyl)-, reaction products with glycidyl tolyl ether on the respiration of activated sewage sludge micro-organisms gave a 3-Hour EC10 value of 29 mg/L and an 3-Hour EC50 value of 66 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
66 mg/L
EC10 or NOEC for microorganisms:
29 mg/L

Additional information