Sodium [4-[[dihydroxy[(2-hydroxy-3,5-dinitrophenyl)azo]phenyl]azo]benzenesulphonato(3-)]cuprate(1-)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 1995 - September 1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: ASTM E 729-780. Standard practice for conducting toxicity tests with fishes, macroinvertebrates, and amphibians

Version / remarks:

1980

Qualifier:

according to guideline

Guideline:

other: OECD 202

Version / remarks:

1981

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material: Everlan Brown EFR

Analytical monitoring:

yes

Test organisms (species):

Cyprinus carpio

Details on test organisms:

- Common name: Common carp
- Source: Wu Sha Tow Fresh Water Fish Culture Demonstration Center, R.O.C.
- Age at study initiation: 60-90 days old
- Length at study initiation: 2-4 cm
- Acclimation period: at least seven days

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

pH:

8.4-8.5

Dissolved oxygen:

73-81%

Details on test conditions:

- Test vessel: Glass tank, 23 cm (id) × 30 cm (h)
- Test volume: 10 L
- Aeration: Gently
- Renewal rate of test solution: 24 hours
- Photoperiod: 12-hours light

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

5.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Table 1. Water quality measurements during the acute toxicity test of Everlan Brown EFR to common carp (Cyprinus carpio)

Nominal concentration (mg/l)	Exposure Time (hr)
	3	24	48	72	96
pH
10	8.4 ± 0.0	8.4 ± 0.0	8.4 ± 0.0	8.5 ± 0.0	8.4 ± 0.0
6.0	8.4 ± 0.0	8.5 ± 0.0	8.4 ± 0.0	8.5 ± 0.0	8.5 ± 0.0
3.6	8.4 ± 0.0	8.4 ± 0.0	8.4 ± 0.0	8.4 ± 0.0	8.5 ± 0.0
2.2	8.4 ± 0.0	8.4 ± 0.0	8.4 ± 0.0	8.4 ± 0.1	8.5 ± 0.0
1.3	8.4 ± 0.0	8.4 ± 0.0	8.4 ± 0.0	8.5 ± 0.0	8.4 ± 0.0
Control	8.4 ± 0.0	8.4 ± 0.0	8.4 ± 0.0	8.4 ± 0.1	8.5 ± 0.0
Dissolved oxygen, % saturation (mg/l)
10	76 (6.3 ± 0.0)	79 (6.5 ± 0.1)	73 (6.0 ± 0.2)	75 (6.2 ± 0.0)	81 (6.7 ± 0.0)
6.0	75 (6.2 ± 0.1)	76 (6.3 ± 0.2)	75 (6.2 ± 0.1)	77 (6.4 ± 0.2)	79 (6.5 ± 0.1)
3.6	75 (6.2 ± 0.0)	77 (6.4 ± 0.1)	75 (6.2 ± 0.1)	76 (6.3 ± 0.0)	77 (6.4 ± 0.2)
2.2	73 (6.0 ± 0.1)	77 (6.4 ± 0.2)	76 (6.3 ± 0.2)	77 (6.4 ± 0.1)	76 (6.3 ± 0.4)
1.3	75 (6.2 ± 0.2)	77 (6.4 ± 0.2)	77 (6.4 ± 0.1)	77 (6.4 ± 0.0)	77 (6.4 ± 0.2)
Control	76 (6.3 ± 0.0)	75 (6.2 ± 0.1)	76 (6.3 ± 0.0)	79 (6.5 ± 0.2)	80 (6.6 ± 0.2)
mg/l calcium as CaCO3 (electrode potential, mV)
10	100 (16 ± 0)	109 (17 ± 0)	109 (17 ± 0)	109 (17 ± 0)	109 (17 ± 0)
6.0	100 (16 ± 0)	109 (17 ± 0)	109 (17 ± 0)	119 (18 ± 0)	109 (17 ± 0)
3.6	109 (17 ± 0)	109 (17 ± 0)	109 (17 ± 0)	119 (18 ± 0)	109 (17 ± 0)
2.2	109 (17 ± 0)	119 (18 ± 0)	109 (17 ± 0)	109 (17 ± 0)	109 (17 ± 0)
1.3	100 (16 ± 0)	119 (18 ± 0)	109 (17 ± 0)	109 (17 ± 0)	109 (17 ± 0)
Control	109 (17 ± 0)	129 (19 ± 0)	119 (18 ± 0)	109 (17 ± 0)	119 (18 ± 0)

Table 2. Survival of common carp (Cyprinus carpio) exposed to Everlan Brown EFR for 96 hours

Nominal Concentration (mg/L)	No. of Survival Fish
	0 hr	3 hr	24 hr	48 hr	72 hr	96 hr
10	30	30	3	2	0	0
6.0	30	30	21	19	18	16
3.6	30	30	27	22	0	0
2.2	30	30	30	30	30	30
1.3	30	29	29	29	29	29
Control	30	30	30	30	30	30

Table 3. The acute toxicity of Everlan Brown EFR to common carp (Cyprinus carpio)

Exposure Time (hr)	LC50 (mg/l)	95% Confidence (mg/l)
		Lower	Upper
3	>100
24	7.0	5.9	8.3
48	5.9	5.1	7.0
72	5.8	5.0	6.8
96	5.6	4.8	6.6

Validity criteria fulfilled:

yes

Conclusions:

According to ASTM E 729-780 and OECD 202 test method, the 96-hr LC50 of Everlan Brown EFR was 5.6 mg/L.

Executive summary:

This test using the procedures outlined in the TACTRI Study Plan for EF3G0083.5 (95AQC212) which is based on the ASTM E 729-780 (ASTM, 1980) and OECD 202 (OECD, 1981). The values of water quality measurements were considered adequate for testing. There was no significant symptom of toxicity noted in this study. Under the conditions of this test, the 96-hr LC50 of Everlan Brown EFR was 5.6 mg/L.

Description of key information

The 96-hr LC50 of Everlan Brown EFR was 5.6 mg/L for common carp (ASTM E 729-780 and OECD TG202).

 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

5.6 mg/L

Additional information

The values of water quality measurements contain pH (8.4-8.5), dissolved oxygen concentrations (73%-81% of air saturated value), and total hardness (110-1299 mg/L as CaCO3) were considered adequate for testing. There was no significant symptom of toxicity noted in this study. Under the conditions of this test, the 96-hr LC50 of Everlan Brown EFR was 5.6 mg/L.