Registration Dossier
Registration Dossier
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EC number: 813-130-5 | CAS number: 4039-86-5
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The ready biodegradability of the test substance was examined according to the OECD 301 B (CO2 evolution, modified Sturm test). After 28 days the test substance was biodegraded by an average of < 10 % CO2/ThCO2, therefore the test substance was found to be not readily biodegradable under the test conditions and furthermore the required pass level for ready biodegradability within a ten days window was not reached.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The ready biodegradability of the test substance was examined in a test (BASF SE, 2015) according to the OECD 301 B (CO2 evolution, modified Sturm test, 1992). The biodegradation of the test substance was followed by exposing it to the active sludge of a domestic waste water treatment plant from Mannheim (Germany). The inoculum was aerated for about 48 hours at 22 +/- 2 °C. The active sludge concentration was 30 mg/L/dry weight. The substance was poorly soluble in water, therefore a water accommodated fraction (WAF) was used. As a reference compound (procedure control), aniline was tested simultaneously under the same conditions. No inhibitory effect on the microorganisms was observed. In the procedure controls, the reference compound aniline was readily biodegraded by an average of 87 % after 14 days of exposure. The validity criteria were met. After 28 days the test substance was biodegraded by an average of < 10 % CO2/ThCO2, therefore the test substance was found to be not readily biodegradable under the test conditions and furthermore the required pass level for ready biodegradability within a ten days window was not reached.
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