o-anisic acid

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Data is from peer-reviewed journal

Data source

Reference

Reference Type:

publication

Title:

Antinociceptive, hypoglycemic and spasmolytic effects of Brickellia veronicifolia

Author:

Francisco Palacios-Espinosa, Myrna De´ ciga-Campos, Rachel Mataa

Year:

2008

Bibliographic source:

Journal of Ethnopharmacology 118 (2008) 448–454

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as below

Principles of method if other than guideline:

Antinociceptive activity of 2-methoxybenzoic acid in mice was studied using Hot-plate test

GLP compliance:

not specified

Test type:

other: No data available

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

o-anisic acid

EC Number:

209-447-8

EC Name:

o-anisic acid

Cas Number:

579-75-9

Molecular formula:

C8H8O3

IUPAC Name:

2-methoxybenzoic acid

Test material form:

solid: crystalline

Details on test material:

- Name of test material : 2-methoxybenzoic acid (2-MBA)
- Molecular formula : C8H8O3
- Molecular weight :152.1482 g/mole
- Smiles notation: COc1ccccc1C(=O)O
- InChl:1S/C8H8O3/c1-11-7-5-3-2-4-6(7)8(9)10/h2-5H,1H3,(H,9,10)
- Substance type: Organic
- Physical state: Solid

Specific details on test material used for the study:

- Name of test material : 2-methoxybenzoic acid (2-MBA)
- Molecular formula : C8H8O3
- Molecular weight :152.1482 g/mole
- Smiles notation: COc1ccccc1C(=O)O
- InChl:1S/C8H8O3/c1-11-7-5-3-2-4-6(7)8(9)10/h2-5H,1H3,(H,9,10)
- Substance type: Organic
- Physical state: Solid

Test animals

Species:

mouse

Strain:

ICR

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Centro UNAM-Harlan (Harlan Mexico, S.A. de C.V.).
- Age at study initiation: No data available
- Weight at study initiation: 20–25 g
- Fasting period before study: Yes, twelve hours before the experiments food was withheld, but animals had free access to drinking water.
- Housing: Animals were housed under standard laboratory conditions as per Mexican Official Norm for Animal Care and Handing (NOM-062-ZOO-1999) and in compliance with international rules on care and use of laboratory animals.
- Diet (e.g. ad libitum): Standard pellet diet, ad libitum
- Water (e.g. ad libitum): Water, ad libitum
- Acclimation period: No data available

ENVIRONMENTAL CONDITIONS
- Temperature (°C): No data available
- Humidity (%):No data available
- Air changes (per hr): No data available
- Photoperiod (hrs dark / hrs light): No data available

IN-LIFE DATES: From: To: No data available

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: saline solution with Tween® 80 (0.05%).

Details on oral exposure:

Details on exposure
VEHICLE
- Concentration in vehicle: 0, 1, 10, 31.6 and 100 mg/kg
- Amount of vehicle (if gavage): No data available
- Justification for choice of vehicle: No data available
- Lot/batch no. (if required): No data available
- Purity: No data available

MAXIMUM DOSE VOLUME APPLIED: No data available

DOSAGE PREPARATION (if unusual): 2-methoxybenzoic acid was suspended with Tween® 80 (0.05%) in saline solution (SS) and then administered orally.

CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: No data available

Doses:

0, 1, 10, 31.6 and 100 mg/kg bw

No. of animals per sex per dose:

Total: 30
0 mg/kg: 6 mice
1 mg/kg: 6 mice
10 mg/kg: 6 mice
31.6 mg/kg: 6 mice
100 mg/kg: 6 mice

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days (or other?): No detailed data available
- Frequency of observations and weighing: Animals were observed before and 30, 60, 90, 120 and 150 min after treatments.
- Necropsy of survivors performed: No data available
- Other examinations performed: other: Antinociceptive activity were observed by using Hot-plate test.

Statistics:

Data are expressed as the mean ±S.E.M. for the number (n=6) of animals in each group. In all cases the results of treated-animal (compounds, extracts and reference drugs) groups were compared with the appropriate control (vehicle) groups. Statistical analysis was performed with one-way ANOVA followed by Dunnett post hoc to determine the source of significant differences where appropriate; *P < 0.05 was considered statistically significant. Prisma Graph-Pad (version 4.0) software was used for statistics and plotting. Analysis of variance was also performed.

Results and discussion

Preliminary study:

No data avaialble

Effect levels

Sex:

male

Dose descriptor:

other: TDLo

Effect level:

10 mg/kg bw

Based on:

test mat.

Remarks on result:

other: Significant increase in noxius stimulation

Mortality:

No data available

Clinical signs:

other: No data available

Gross pathology:

No data available

Other findings:

Antinociceptive activity:
A significant dose dependent increase was observed in pain response of treated mice when test by using hot plate test as compare to control.

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Conclusions:

TDLo was considered to be 10 mg/kg when male ICR (Mus domesticus) mice were treated with 2-methoxybenzoic acid (2-MBA) orally.

Executive summary:

Acute oral toxicity study was conducted to evaluate the antinociceptive effect of the test compound 2-methoxybenzoic acid (2-MBA) in Male ICR (Mus domesticus) mice in the concentration of 0, 1, 10, 31.6 and 100 mg/kg. Results show dose dependent increase in pain response of treated mice as compared to control. Therefore, TDLo was considered to be 10 mg/kg when male ICR (Mus domesticus) mice were exposed to 2-methoxybenzoic acid (2-MBA) orally.