Trifluoroacetic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: A reliable and scientifically sound guideline study referring to a read-across substance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Sodium trifluoroacetate

EC Number:

220-879-6

EC Name:

Sodium trifluoroacetate

Cas Number:

2923-18-4

Molecular formula:

C2HF3O2.Na

IUPAC Name:

sodium trifluoroacetate

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material:
PHYSICO-CHEMICAL PROPERTIES
- Molecular weight: 136 g/mol
- Water solubility: 625 g/L at 25°C
- Appearance: white powder

Based on the molecular weights, a concentration of 1200 mg/L sodium trifluoroacetate/L corresponds to 1000 mg trifluoroacetate anion/L.

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0 and 1200 mg/L (nominal) in ISO water
- Sampling: 0h and 96h after test initiation
- Sample storage conditions before analysis: the samples were stored at cool and dark before analysis

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: Ruinemans, Montfoort, The Netherlands (commercial supplier)
- Length at study initiation (mean): 2.3 - 3.4 cm
- Weight at study initiation (mean): 0.23 g
- Feeding during test: no


ACCLIMATION
- Conditions: the zebra fishes were held in plastic containers of 40 x 60 X 40 cm containing ca. 80 L reconstitued water (ISO), continuously filtered
- Type of food: trout feed and water fleas

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Test temperature:

21.0 - 22.8°C

pH:

7.6 - 7.9

Dissolved oxygen:

8.3 - 8.7 mg/L

Nominal and measured concentrations:

Nominal: 1200 mg/L and control
Measured (mean): 1210 mg/L (at test start) and 1205 mg/L (at test end)

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria
- Fill volume: 4L ISO water
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: reconstitued water (ISO)
Deionised water (LRS 1322 830 78115), 294 mg/L CaCl2*2H2O, 123 mg/L MgSO4*7H2O, 64 mg/L NaHCO3, 5.8 mg/L KCl
(pH = 7.8; hardness (CaCO3 = ca. 250 mg/L))
- Intervals of water quality measurement: at the beginning (0h) and at the end of the study (96h)

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h/light; 8h/dark

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0, 100, 300, 1000 mg/L
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

Results with reference substance valid? yes
- EC50 (96h): 142 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes