Registration Dossier
Registration Dossier
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EC number: 289-632-8 | CAS number: 89958-10-1 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Bulnesia sarmienti, Zygophyllaceae.
Endpoint summary
Administrative data
Description of key information
In order to assess the biotic degradation, a ready biodegradability test was performed which allows the biodegradability to be measured in an aerobic aqueous medium.
The ready biodegradability was determined in the Closed Bottle test performed according to slightly modified OECD (TG OECD 301 D), EU and ISO Test Guidelines, and in compliance with the OECD principles of Good Laboratory Practice. The test substance did not cause a reduction in the endogenous respiration. The test substance is therefore considered to be non-inhibitory to the inoculum. The validity criteria for the test were met. Guaiacwood oil was biodegraded by 80% at day 28 in the Closed Bottle test. The substance is an UVCB, so the 14-day window is not relevant for ready biodegradability classification. Hence, this substance should be classified as readily biodegradable.
Prior to the key study summarised above, the ready biodegradability of Guaiacwood oil was investigated in a series of Closed Bottle tests according to OECD TG301D, not under GLP. The goal of this study was to investigate the influence of the source of inoculum and test substance dose on the biodegradability of the substance. Tests were performed with activated sludge and river water as inocula, and with test substance concentrations of 1 and 2 mg/L.
Biodegradation of ≥60% was found within two week with both activated sludge and river water. The substance therefore meets the criteria for ready biodegradable substance with both inocula. For the final GLP test it was recommended to use river water as inoculum.
Additional information
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