Silicon boride oxide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed according to OECD Guideline and to Good Laboratory Practice

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Boron silicate

EC Number:

603-157-9

Cas Number:

12676-29-8

Molecular formula:

H7 B7 Si57 O128

IUPAC Name:

Boron silicate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Purity: > 90%
Batch No.: 97731275L0

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

The test species of Zebra fish, Brachydanio rerio (Batch No: F20120115, Hatch date: Aug, 2011 ), were obtained as fry from a commercial fish supplier of Guangzhou Hongfa Aquatic Animals Culture Company in China.
The wet-weight of the fish used to the test was 0.30 g in average, with an average length of 2.85 cm.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

120 mg/L CaCO3

Test temperature:

23 °C

pH:

7.40 - 7.65

Dissolved oxygen:

89% to 99% of the air saturation

Nominal and measured concentrations:

Nominal concentrantrations of 150 mg/L (measured concentration 3.09 mg/L) and 100 mg/L

Details on test conditions:

In the Iimit test, W AFs (water accommodated fractions) solutions with a nominal concentration of 100 mg/L and 150 mg/L were prepared by adding
appropriate amounts of the test substance to the test medium in glass aspirator bottles with appropriate size.
The mixing aspirator bottle was stoppered and the aqueous test substance rnixtu re was stirred for 24 hrs on a magnetic stirplate and a Telfon
stirbar at room temperature (about 23- 25°C). The vortex height was set at least 10% of the static liquid depth. At the end of the 24 h, stirring was
stopped and the test substance was allowed to settle down. The mixture was allowed to settle for 1 hour at room temperature, and undissolved test
substance deposited on the bottom. Then the aqueous portion removed through the outlet of the aspirator stirring bottle was just the W AF solutions (100 mg/L and 150 mg/L) of the test substance.
Light: 16 hours photoperiod daily (light intensity: 1000 to 1500 Iux)

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

>= 150 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)