complex reaction mass of Chinese gum rosin post reacted with acrylic acid

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.6800 (Modified Activated Sludge, Respiration Inhibition Test for Sparingly Soluble Chemicals)

Test guideline 3

Qualifier:

according to guideline

Guideline:

other: Biodegradation activated sludge respiration inhibition test of commission directive 87/302/EEC

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Hydrogenated reaction products of rosin with acrylic acid

Cas Number:

144413-22-9

IUPAC Name:

Hydrogenated reaction products of rosin with acrylic acid

Test organisms

Test organisms (species):

activated sludge

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

3 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Any other information on results incl. tables

In the single test vessel prepared using a preliminary solution in auxiliary solvent to give a test concentration of 1.0 mg/l (the limit of water solubility), no inhibition of the respiration rate of activated sewage sludge was observed and hence the 3-hour EC50 value

was determined to be greater than 1.0 mg/l. Correspondingly the No Observed Elfect Concentration (NOEC) determined from this vessel was equal to 1.0 mg/l. These results show that at the limit of water solubility of the test material, the test material has no effect

on the respiration rate of activated sewage sludge.

The effect of the test material on the respiration rate of activated sewage sludge at concentrations in excess of the water solubility level of the test material was also assessed using a single test concentration of 1000 mg/l prepared by direct dispersion of the test

material in test water with the aid of ultrasonication. At a test concentration in excess of the water solubility level of the test material inhibition of respiration of the activated sewage sludge did not occur and the 3-hour EC50 value was determined to be greater than

1000 mg/l. Correspondingly the NOEC was determined to be equal to 1000 mg/l. These results show that at concentrations in excess of the water solubility level of the test material, the test material does not have an effect on the respiration rate of activated sewage sludge.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The effect of the test material on the respiration of activated sewage sludge mieraorganisms gave a 3-Hour EC50 of greater than er equal to 1000
mg/l prepared using a direct dispersion in water. Correspondingly the No Observed Effect Concentration (NOEC) was equal to 1000 mg/I. In the
single test vessel prepared using a preliminary solution in auxiliary solvent to give a test concentration of 1.0 mg/I (limit of water solubility), no inhibition
of the respiration rate of activated sewage sludge was observed and hence the 3-Hour EC50 value was determined to be greater than 1.0 mg/I.
Correspondingly the No Observed Effect Concentration determined from this vessel was equal to 1.0 mg/I. These results show that at the limit of
water solubility no effect on the respiration rate of activated sewage sludge micro-organisms was observed.

Executive summary:

The effect of the test material on the respiration of activated sewage sludge mieraorganisms gave a 3-Hour EC50 of greater than er equal to 1000 mg/l prepared using a direct dispersion in water. Correspondingly the No Observed Effect Concentration (NOEC) was equal to 1000 mg/l. In the single test vessel prepared using a preliminary solution in auxiliary solvent to give a test concentration of 1.0 mg/l (limit of water solubility), no inhibition of the respiration rate of activated sewage sludge was observed and hence the 3-Hour EC50 value was determined to be greater than 1.0 mg/l. Correspondingly the No Observed Effect Concentration determined from this vessel was equal to 1.0 mg/l. These results show that at the limit of water solubility no effect on the respiration rate of activated sewage sludge micro-organisms was observed.