Phenethyl isovalerate

Endpoint summary

Additional information

Short term Fish toxicity:

Based on the Weight of evidence approch for the target substance Phenethyl isovalerate studies are summarized as fallowed :

Based on theEPI Suite ECOSAR version 1.10, the 96 hours LC50 was estimated to be 2.872 mg/L on fish for substance Phenethyl isovalerate with mortality effects.

And in second study which is based on theDanish QSAR database, the short term toxicity in fish for 96 hours LC50 was estimated to be 2.38025 mg/L for substance Phenethyl isovalerate with mortality effects.

But the partition coefficient (log Kow ) value for test substance not≥4 it is found to be in the range 3.71 to 3.9 thus not fulfil the classification criteria of CLP and therefore the target chemical not consider for the aquatic classification.

Above conclusion also support by read across chemical (Cas no.140-11-4) study from peer reviewed journal(U.S.Fish.Wildl.Serv., Sp.Sci.Rep; 124 p., 1963)i.e Short term toxicity study to Cyprinus carpio was carried out for 44 hrs. Test was performed under flow through conditions. After exposure of test animal to different dose conc. i.e, at 68, 130 and 146 mg/l of test chemical benzyl acetate, no effects were observed on mobility of the test animal. Thus, the NOEC value was consider to be 146 mg/l respectively.

Overall all studies concluded that the test substance Phenethyl isovalerate is non toxic to aquatic environment thus not consider for the further classification.

Short term toxicity to aquatic invertebrates

Aim of this study was to assess the short term toxicity of Phenethyl isovalerate

to aquatic invertebrates daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs.

 

The stock solution 100 g/L was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. It was not possible to test at higher concentration because of limited solubility of tested sample (Changable sentence according to the chemical).0.5,1,2,4,8 mg/Lnominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 

The median effective concentration (EC50) for the test substance Phenethyl isovalerate

, in Daphnia magna was determined to be 6.4 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, indicates that the substance is likely to be hazardous/ non-hazardous to aquatic invertebrates and can be be classified as aquatic chronic 2 category as per the CLP criteria.

Toxicity to aquatic alga and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201.The test solution 100 mg/l was prepared by dissolving yellow powder in OECD growth medium. Effects on the growth rate of the organism were studied (Changable). Various concentration were used.

With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.

The median effective concentration (ErC50) for the test substance2-phenylethyl 3-methylbutanoate, in algae was determined to be 13.4 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, indicates that the substance is likely to be hazardous to aquatic algae and can be be classified as aquatic chronic 3 category as per the CLP criteria.