Registration Dossier
Registration Dossier
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EC number: 257-854-4 | CAS number: 52333-30-9
Endpoint summary
Administrative data
Description of key information
Acid Red 260 can be regarded as not acute toxic to aquatic species.
Additional information
Short term toxicity tests with Acid Red 260 are available for all the three tropic levels (fishes, invertebrates and aquatic plants). In an acute fish toxicity test, FAT 21021/A was observed to have 48 h TL50 about 400 mg/L. In the Daphnia magna study, no immobilization and abnormal behavior of the Daphnia was observed in the controls and in the test concentration up to 20.5 mg/L (nominal 100 mg/L). Therefore, the NOEC and EC0 value after 48 hours were determined to be 20.5 mg/L. The EC 100 could not be determined. The EC 50 value after 24 and 48 hours could not be calculated, but was clearly higher than 40.4 mg/L. In conclusion the EC 50 value of FAT 21021/D was above the limit of solubility of the test substance in the test medium. Acid Red 260 is a dark color dye which when mixed in test medium will impart dark color. Due to light absorption, colored substances such as dyes can inhibit algal growth, which is not the result of a toxic action. This shading effect can confound measures of chemical toxicity and thus impair the risk assessment. In an effort to avoid this problem Lemna gibba (Duckweed) Growth Inhibition test (Static) was conducted. In a GLP-compliant OECD Guideline 221 study, Lemna gibba (Duckweed) exposed to the test item FAT 21021 recorded: 7 day ErC50 based upon the frond number is >100mg/L. 7 day EyC50 based upon the frond numbers is >100 mg/L. 7 day ErC50 based upon the frond dry weight is >100 mg/L. 7 day EyC50 based upon the frond dry weight is >100 mg/L. Toxicity of FAT 21021/D to activated sludge was determined in a 3-hours respiration inhibition test according to OECD Guideline No. 209 and performed in compliance with GLP. The results revealed the 3h IC50 of FAT 21021/D was greater than >320 mg/L. Based on these results,Acid Red 260 can be regarded as not acute toxic to aquatic species.
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