Chromate(2-), [2,4-dihydro-4-[(2-hydroxy-5-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)][3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulfonato(3-)]-, disodium

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2004

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 20010/D
Purity: 95 %

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: Zebra fish

Details on test organisms:

No of fish: 7 per concentration.

Test type:

static

Water media type:

not specified

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

21.4 - 21.6 °C

pH:

7.9 - 8.1

Dissolved oxygen:

97 - 99 %

Nominal and measured concentrations:

Nominal concentration: 1, 10 and 100 mg/L

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

< 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

< 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Mortality of Zebra fish: Number of dead fish after 2 -4h, 24, 48 , 72 and 96 h

Conc. mg/L (Nominal)	No. of Fish	Number of dead fish after
		2 - 4 h	24 h	48 h	72 h	96 h
Blank	7	0	0	0	0	0
1.0	7	0	0	0	5	6
10	7	7	7	7	7	7
100	7	7	7	7	7	7

Validity criteria fulfilled:

yes

Conclusions:

The LC50 (96 h) of FAT 20010/D to the Zebra fish was found to be <1.0 mg/L.

Executive summary:

Acute toxicity of test substance FAT 20010/D was assessed by following internal method comparable to today’s standard testing procedure published by the OECD within Guideline for Testing of Chemicals 203: Fish, Acute Toxicity Test, adopted by the Council on 17th July 1992. The nominal concentrations used were 1, 10, 100 mg/L. 7 zebra fish were used per concentration. The following 96 h LC values were noted when Zebra fish were exposed to test substance FAT 20010/D in a static system:

LC0 (96 h): <1.0 mg/L

LC50 (96 h): <1.0 mg/L

LC100 (96 h): 10 mg/L

Description of key information

96 h LC50 value to fresh water Zebra fish <1.0 mg/L. The test substance FAT 20010 can be regarded as very toxic to fish.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 mg/L

Additional information

In an 96 h acute fish toxicity test, the test substance FAT 20010 was assessed by following internal method comparable to today’s standard testing procedure published by the OECD within Guideline for Testing of Chemicals 203: Fish, Acute Toxicity Test, adopted by the Council on 17th July 1992. FAT 20010 was observed to have 96 h LC50 to Zebra fish <1.0 mg/L. Another study conducted in 1973 reported 48 h TL50 of 6 mg/l. Based on these findings the test substance FAT 20010 can be regarded as very toxic to fish.