Benzoic acid, 2,3,4,5-tetrachloro-6-cyano-, methyl ester, reaction products with 2-methyl-1,3-benzenediamine and sodium methoxide

Repeated dose toxicity: dermal

Administrative data

Endpoint:

short-term repeated dose toxicity: dermal

Type of information:

experimental study

Adequacy of study:

other information

Study period:

1964

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Non-GLP non-guideline study, available as unpublished report, notable limitations in design and/or reporting.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1964

Report date:

1964

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

In a repeated dose toxicity study 10 rats (5/sex) were exposed daily, during 4 weeks (Saturdays and Sundays excluded), to Irgazingelb 2 GLT: 5% suspension with Arabic gum (application volume 0.4 cm³/animal). To prevent licking, the animals were kept in a narrow grating cage during 3 hours after application, subsequently the application site (ca. 20 cm²) was wiped with a wet sponge.

GLP compliance:

not specified

Remarks:

prior to GLP implementation

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Mixture of octachloro, monomethoxy-heptachloro and bismethoxy-hexachloro derivatives of 3,3'-[(2-methyl-1,3-phenylene)diimino]bis[2,3-dihydro-1H-isoindol-1-one]

EC Number:

600-735-2

Cas Number:

106276-79-3

Molecular formula:

Mixture of C23H8Cl8N4O2, C24H11Cl7N4O3, C25H14Cl6N4O4

IUPAC Name:

Mixture of octachloro, monomethoxy-heptachloro and bismethoxy-hexachloro derivatives of 3,3'-[(2-methyl-1,3-phenylene)diimino]bis[2,3-dihydro-1H-isoindol-1-one]

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 124 - 139 g, average 133 g

Administration / exposure

Type of coverage:

not specified

Vehicle:

other: aqueous suspension with Arabic gum

Details on exposure:

TEST SITE
- Area of exposure: ca. 20 cm²
- Amount(s) applied (volume or weight with unit): 0.4 cm³/animal
- Application: 1 x per day, durning 4 weeks, always on the same skin area (saturdays and sundays excluded)
- Shavings or clipplings: depilated back skin

REMOVAL OF TEST SUBSTANCE
- Washing (if done): wiped with a wet sponge
- Time after start of exposure: 3 hours

TEST MATERIAL / VEHICLE
- Concentration (if solution): 5% suspension with Arabic gum

USE OF RESTRAINERS FOR PREVENTING INGESTION: yes
To prevent licking, the animals were kept during 3 hours after administration in a narrow grating cage.

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

4 weeks

Frequency of treatment:

Daily, excluding saturdays and sundays.

No. of animals per sex per dose:

5/sex

Control animals:

not specified

Examinations

Observations and examinations performed and frequency:

SKIN OBSERVATIONS: Yes
Assessment of skin observations checked respectively 24 h after or before the next application.

BODY WEIGHT: Yes
At start and termination of the experiment

RESORPTIVE TOXICITY: Yes

Results and discussion

Results of examinations

Details on results:

- Body weight at termination of experiment: 183 - 246 g, average 221g
- 10/10 without without findings
- No local Irritation
- No resorptive toxicity

Effect levels

Dose descriptor:

NOAEL

Remarks on result:

not determinable

Remarks:

no NOAEL identified

Target system / organ toxicity

Critical effects observed:

not specified