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EC number: 247-660-8 | CAS number: 26401-35-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions, not GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- adopted 7 June 1984
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: test substance in concentrations of 100 and 1000 mg/l were moderately stirred in algal medium for 24 h at room temperature, after this the undissolved materials were removed by sterile filtration (0.45µm), the resulting water accommodated fractions (WAFs) were used in the test
- Control: algal medium - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: 86.81. SAG
- Source: from the culture collection in Göttingen, Germany
- Method of cultivation: axenic slope culture, no further details mentioned, preculture for the test: 100 ml flask containing 50 ml of sterile algal medium (according to guideline), inoculated with cell material from axenic culture, exponentially growing culture used for the test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- 2 concentrations tested: 100 and 1000 mg/l as WAF
- Test temperature:
- 22 +/- 0.2 °C
- pH:
- 7.7 - 8.0
- Nominal and measured concentrations:
- 100, 1000 mg/l nominal, tested as WAFs
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml flasks, all-glass, with 100 ml of test medium
- Initial cells density: 1x10E4 - 2x10E4 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 4
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 8500 - 9000 lux, warm white fluorescent tubes
EFFECT PARAMETERS MEASURED :
- Determination of cell concentrations: counting chamber to define the starting cell density, spectrophotometer (at 680 nm) after 24 , 48 and 72 h of exposure
- Chlorophyll measurement: no
- Other: temperature (in 1 control flask at the start and at the end of the test), pH (in 1 control flask after sterilization before the test and in all test vessels at the end of the test), observations of mis-shapen cells and cell debris after 24, 48 and 72 h of exposure - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- as water accommodated fraction
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- as water accommodated fraction
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control: yes
- Observation of abnormalities: none
- Unusual cell shape: no - Results with reference substance (positive control):
- periodically conducted in the testing laboratory, no further details mentioned
- Validity criteria fulfilled:
- yes
- Remarks:
- all values (pH, temperature) were in the accepted ranges, cell concentarion in control cultures increased more than 16 fold within 3 days
- Conclusions:
- The EL50 based on the growth rate is > 1000 mg/l nominal concentration, the NOEL based on the growth rate is >= 100 mg/l nominal concentration.
- Executive summary:
The median growth inhibiting concentration and the no-observed effect concentration based on growth rates of Diisotridecyl adipate to the green alga Scenedesmus subspicatus were investigated over a period of 72 h. The only applied concentrations were 100 and 1000 mg/l.
3 test vessels were exposed to each test concentration.
Due to the limited water solubility of the test substance, Diisotridecyl adipate in a concentrations of 100 and 1000 mg/l, respectively, were moderately stirred in algal medium for 24 h at room temperature. After this incubation time, the undissolved materials were removed by sterile filtration. The resulting water accommodated fractions were used in the test. No analysis of the test substance concentrations was conducted.
At the loading rate of 100 mg/l no significant growth inhibition compared to the untreated controls was observed. At the loading rate of 1000 mg/l a mean growth inhibition of 10.6% compared to the untreated control was observed.
Based on the result of the study it can be concluded that the EL50 (24 -72 h) of Diisotridecyl adipate based on the growth rate is > 1000 mg/l nominal concentration and the NOEL based on the growth rate is >= 100 mg/l nominal concentration.
Reference
Table #1: Cell concentrations after 24, 48 and 72 h of exposure
Loading rate [mg/l] | Cell concentration (measured photometrical extinction at 680 nm) mean values | |||
0 h | 24 h | 48 h | 72 h | |
Control | 0.013 | 0.042 | 0.170 | 0.625 |
100 | 0.013 | 0.047 | 0.168 | 0.643 |
1000 | 0.013 | 0.054 | 0.178 | 0.608 |
Table #2: Average specific growth rates between 24 and 72 h
Loading rate [mg/l] | Average specific growth rate x 0.01 | Inhibition [%] | Standard deviation [%] |
Control | 5.618 | 0.0 | 3.1 |
100 | 5.443 | 3.0 | 2.1 |
1000 | 5.200 | 10.6 | 3.0 |
Description of key information
EL50(72h) > 1000 mg/L (nominal, loading rate) for growth rate of Desmodesmus subspicatus (OECD 201)
NOEL(72h) >= 100 mg/L (nominal, loading rate) for growth rate of Desmodesmus subspicatus (OECD 201)
Key value for chemical safety assessment
Additional information
Based on the result of the algae study it can be concluded that the ErL50 (24 -72 h) of diisotridecyl adipate based on the growth rate is > 1000 mg/l nominal loading rate and the NOErL based on the growth rate is ≥ 100 mg/l nominal loading rate (Häner/Panolin, 1998). The effect levels are far above the limit of water solubility of diisotridecyl adipate.
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