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EC number: 246-836-1 | CAS number: 25321-14-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian cell study: DNA damage and/or repair
- Remarks:
- Type of genotoxicity: DNA damage and/or repair
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Reliability as cited in OECD SIDS Dinitrotoluene (isomers mixture) CAS No.: 25321-14-6, 2004. Guideline study without detailed documentation
Data source
Reference
- Reference Type:
- publication
- Title:
- Measurement of unscheduled DNA synthesis and S-Phase synthesis in rodenthepatocytes following in vivo treatment: Testing of 24 compounds.
- Author:
- Mirsalis, J.C. et al.
- Year:
- 1 989
- Bibliographic source:
- Environ Molec Mutagen 14, 155-164
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 486 (Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo)
- Deviations:
- yes
- Remarks:
- only pooled data shown; composition of test substance not described in detail
- GLP compliance:
- no
- Type of assay:
- unscheduled DNA synthesis
Test material
- Reference substance name:
- Dinitrotoluene
- EC Number:
- 246-836-1
- EC Name:
- Dinitrotoluene
- Cas Number:
- 25321-14-6
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- Reaction mass of 1-methyl-2,4-dinitrobenzene and 2-methyl-1,3-dinitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): technical-grade dinitrotoluene
- Isomers composition: DNT; 76.5% 2,4-DNT, 18.8% 2,6-DNT, 4.7% other isomers
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Fischer 344
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Simonsen Laboratories, Gilroy, CA and Hilltop Laboratory Animals, Chatsworth, CA
- Weight at study initiation: 180 - 300 g
- Housing: Three per cage in polypropylene cages with hardwood-chip bedding
- Diet (e.g. ad libitum): Purina Rodent Chow #5001 (Ralston Purina Co., St. Louis); ad libitum
- Water (e.g. ad libitum): deionized, 0.5 µm charcoal filtered tap water; ad libitum
ENVIRONMENTAL CONDITIONS
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle(s)/solvent(s) used: corn oil; no data on the solvents of positive controls
- Duration of treatment / exposure:
- single bolus
- Frequency of treatment:
- once
- Post exposure period:
- 2 and 12 hours
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 35, 125 and 250 mg/kg bw
Basis:
actual ingested
- No. of animals per sex per dose:
- 3; only two animals at 250 mg/kg bw
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- 2-acetylaminofluorene; dimethylnitrosamine
- Route of administration: oral: gavage
- Doses / concentrations: 2-AAF: 50 mg/kg bw (12 h); DMN: 10 mg/kg bw (2 h)
Examinations
- Tissues and cell types examined:
- primary rat hepatocyte cultures
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION: Doses were selected based approximately on the oral LD50 of the compound and were generally selected as 80%, 40%, and 10% of the LD50. The acute LD50 was never exceeded.
TREATMENT AND SAMPLING TIMES ( in addition to information in specific fields): 2 and 12 h
DETAILS OF SLIDE PREPARATION: Cells were allowed to attach to coverslips.
METHOD OF ANALYSIS: 50 morphologically unaltered cells were counted per slide. Three slides were scored for each animal or concentration for a total of 150 cells per animal. UDS was measured by quantitative autoradiography as net grains (NG) per nucleus. The percentage of cells undergoing repair (%IR) was determined as the percent of those cells exhibiting 5 or more NG.
OTHER: - Evaluation criteria:
- Compounds were considered negative if the NG of all dose groups was a negative number and the %IR was less than 10%. Compounds were considered positive if the average NG of any dose group exceeded 0 NG. Compounds with negative NG values, but %IR values greater than 10% were considered equivocal.
- Statistics:
- The mean and the standard error of the mean were calculated.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- positive
- Toxicity:
- not specified
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- valid
Any other information on results incl. tables
Dose [mg/kg] |
Time [h] |
NG |
n |
%IR |
|
0 (corn oil) |
2 |
-6.4 ± 2.9 |
2 |
1 ± 0 |
|
0 (corn oil) |
12 |
-5.6 ± 0.4 |
52 |
2 ± 0 |
|
DNT |
35 |
2 |
-11.2 ± 1.7 |
3 |
4 ± 0 |
12 |
-5.5 ± 1.2 |
3 |
3 ± 1 |
||
125 |
12 |
17.4 ± 3.1 |
3 |
77 ± 7 |
|
250 |
12 |
31.0 ± 7.8 |
2 |
89 ± 5 |
|
2-AAF |
50 |
12 |
22.9 ± 1.7 |
52 |
85 ± 2 |
DMN |
10 |
2 |
41.8 ± 3.4 |
31 |
90 ± 2 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): positive
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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