Phosphoryl trichloride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Older guideline-comparable study reported in summary form

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

The test method is comparable to the withdrawn OECD TG 401

GLP compliance:

no

Remarks:

: the study pre-dates GLP

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Phosphoryl trichloride

EC Number:

233-046-7

EC Name:

Phosphoryl trichloride

Cas Number:

10025-87-3

Molecular formula:

Cl3OP

IUPAC Name:

phosphoroyl trichloride

Test material form:

liquid: volatile

Details on test material:

The test material 'MCTR-191-77' is stated to be a colourless, transparent, viscous and volatile liquid.

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

A total of 50 male and female rats weighing between 200 and 300 grams, were fasted for approximately 24 hours prior to the administration of the test material.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

The test material sample 'MCTR-191-77' (=phosphoryl trichloride) was administered on a mg/kg basis as a 10% solution in corn oil.

Doses:

The dose levels of the test substance included in the study were 50, 100, 200, 300 and 400 mg/kg bw.

No. of animals per sex per dose:

Ten animals (male and female; not speciifed further)

Control animals:

no

Details on study design:

The animals were observed at 1, 3, 6, 24, 72 and daily thereafter for a total of 14 days if survival allowed and then killed, autopsied and observed for gross pathological organ changes.

Statistics:

None required

Results and discussion

Preliminary study:

None

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

110 mg/kg bw

Based on:

test mat.

95% CL:

> 91 - < 129

Remarks on result:

other: Calculated according to the method of Miller & Tainter, 1944.

Mortality:

Two deaths were recorded after rats were administered with the 50 mg/kg dose, 4 deaths were recorded following the 100 mg/kg dose, 9 deaths were recorded following the 200 mg/kg dose, 9 deaths were recorded following the 300 mg/kg dose and 10 deaths were recorded following the 400 mg/kg dose

Clinical signs:

other: Decreased locomotor activity, piloerection, ptosis, suspected blood around the eyes, loss of righting reflex and death. Chronic pulmonary disease was revealed at 50, 100 and 200 mg/kg body weight. Normal body activity returned within 7 days in all surviv

Gross pathology:

Autopsies revealed lung fused to the rib cage in rats adminstered with the 50 mg/kg dose level. At 100 mg/kg there appeared to be lung fused to the rib cage and filled with a white mass, with irregular thickening on the cardiac mucosa

Other findings:

None

Any other information on results incl. tables

Distribution of Mortality

Dose (mg/kg bw)	Deaths	Hour			Observation Day
		1	3	6	1	2	3	4	5	6	7	8	9	10	11	12	13	14
50	2/10	0	0	0	1	0	0	0	1	0	0	0	0	0	0	0	0	0
100	4/10	0	0	0	1	2	0	0	0	1	0	0	0	0	0	0	0	0
200	9/10	1	7	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
300	9/10	4	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
400	10/10	4	5	0	1	-	-	-	-	-	-	-	-	-	-	-	-	-

 

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

The acute oral LD50 of phosphoryl trichloride in the rat was found to be 110 mg/kg bw under the conditions of this study. Based on these results, 'MCTR-191-77' is classified with Acute Tox. 3 (H301, Toxic if swallowed) according to GHS criteria.

Executive summary:

Groups of ten male and female Sprague-Dawley rats were gavaged with single doses of phosphoryl trichloride (10% in corn oil) at dose levels of 50, 100, 200, 300 or 400 mg/kg bw and observed for 14 days. Gross necropsy was performed on all animals.

 

Thirty-four deaths were recorded following administration of the test substance, with the majority of deaths occurring in the higher dose level groups. The following signs of toxicity were observed: decreased locomotor activity, piloerection, ptosis, suspected blood around the eyes, loss of righting reflex and death.  Chronic pulmonary disease was revealed at dose levels of 50, 100 and 200 mg/kg bw.  Normal activity returned within seven days in all surviving animals. Autopsies revealed lung fused to the rib cage in rats administered 50 mg/kg bw. At 100 mg/kg bw there appeared to be lung fused to the rib cage and filled with a white mass, with irregular thickening of the cardiac mucosa

The acute oral LD50 in the rat was calculated to be 110 mg/kg bw under the conditions of this study.