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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Description of key information

The study from Kärmas (2004) is retained as the key study for this endpoint, with a NOEC of 13.3 mg/L and an EC50 of 29 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
29 mg/L
EC10 or NOEC for microorganisms:
13.3 mg/L

Additional information

Two studies were conducted to assess the toxicity of hydrogen sulphide to microorganisms. The one from Kärmas (2004) was conducted according to ISO 8192 on activated sludge of a predominantly industrial sewage, but some information is lacking, like the duration of the test. An EC50 of 29 mg/L was determined (as total sulphide), based on inhibition of oxygen consumption.

In a second study from Küster et al. (2005) is a non-standard test, where the luminescent bacterium Vibrio fischeri was exposed for 30 minutes to hydrogen sulphide, synthesized electrochemically. The measured EC50-30min measured was 9.4 mg/L (as H2S).

Vibrio fischeri is a marine bacterium, with limited relevance for STP functioning. Therefore, the Kärmas study is retained as the key study for this endpoint, with an EC50 of 29 mg/L.