Registration Dossier
Registration Dossier
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EC number: 201-553-2 | CAS number: 84-69-5
Additional toxicological data
Administrative data
- Endpoint:
- additional toxicological information
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, non-guideline study. Available as a publised report. Acceptable with restrictions.
Data source
Reference
- Reference Type:
- publication
- Title:
- Differential effects of phthalate esters on transciptional activities via human estrogen receptors α and ß, and androgen receptor
- Author:
- Takeuchi S, Iida M, Kobayashi S, Jin K, Matsuda T, Kojima H
- Year:
- 2 005
- Bibliographic source:
- Toxicology 210, 223-233
Materials and methods
- Type of study / information:
- Estrogenic and androgenic activity of diisobutyl phthalate.
- Principles of method if other than guideline:
- Highly sensitive gene reporter assays used to determine the endocrine disrupting potential of the test substance via a number of different routes.
Plasmids were prepared as per Kojima et al. 2003, 2004 - GLP compliance:
- not specified
Test material
- Reference substance name:
- Diisobutyl phthalate
- EC Number:
- 201-553-2
- EC Name:
- Diisobutyl phthalate
- Cas Number:
- 84-69-5
- Molecular formula:
- C16H22O4
- IUPAC Name:
- 1,2-bis(2-methylpropyl) benzene-1,2-dicarboxylate
- Details on test material:
- Purity: >99.0%
Source: Wako Pure Chemical Industriues, Ltd.
Constituent 1
Results and discussion
Any other information on results incl. tables
Diisobutyl phthalate was observed to have the following effects (> 20% inhibitory effect with 10^-5 M DIBP)
Agonistic activity via ERα [REC20 = 6.1*10^-6 M]
Antagonsitic activity via ERß [RIC20 = 6.2*10^-6 M]
Antiandrogenic activities in hAR-transactivation assay
Negative results were observed for:
Agonistic activity via ERß
Antagonsitic activity via ERα
Androgenic activity in AR assay
Cytotoxicity or suppression of ß-Gal activity
Applicant's summary and conclusion
- Conclusions:
- Diisobutyl phthalate was found to exhibit ERα-estrogenic activity and antiandrogenic activity.
- Executive summary:
In a non-GLP, non-guideline study Diisobutyl phthalate was found to was found to exhibit ERα-estrogenic activity and antiandrogenic activity.
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