2-methylbutyl acrylate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Read-across of data for an analogous chemical. The target chemical and the source chemical are very closely related alkyl acrylate compounds. They differ only by the addition of a methyl group in the butyl portion of the molecule. Both molecules are expected to be metabolized via the same hydrolysis and enzymatic pathways. The data from the source chemical are experimental results from well documented studies which meet basic scientific principles and are considered to be acceptable with restrictions. Comparable to guideline with acceptable restrictions (dose level not up to cytotoxic range; neither Escherichia coli strain WP2 uvrA pKM101a nor S. typhimurium strain TA102 were employed in this study for the detection of oxidising mutagens and cross-linking agents)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Principles of method if other than guideline:

The study was conducted according to the method described by Ames (1975) with minor modifications.

Ames BN et al. (1975). Mut. Res. 31: 347-364

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Butyl acrylate

EC Number:

205-480-7

EC Name:

Butyl acrylate

Cas Number:

141-32-2

IUPAC Name:

butyl acrylate

Details on test material:

- Name of test material (as cited in study report): Butyl acrylate
- Physical state: liquid
- Analytical purity: approximately 99.6 %
- Impurities (identity and concentrations): 0.4% butylpropionate, 15 ppm methyletherhydroquinone

Method

Target gene:

Histidin auxotrophy

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

Aroclor 1254 induced Sprague-Dawley-rat liver S-9 mix

Test concentrations with justification for top dose:

3.15 to 1000 nL per plate (2.84, 9.0, 28.4, 90.0, 283.5 and 900.0 µg per plate) Calculation (µg/plate) based on density of the test substance (0.9 g/mL).

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: with S9: 2-Aminoanthracene, Benzo(a)pyrene, 3-Methylcholanthrene and trans-N-Methyl-4-Aminostilbene; without S9: N-Methyl-N-Nitro-N-Nitrosoguanidine, Benzo(a)pyren-4-5-oxide

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation)

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

not applicable

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Mean number of revertants with metabolic activation

Test substances	Dose / plate (µg)	TA1535	TA1537	TA98	TA98 TCPO*	TA100
Butyl acrylate	2.84	35	23	68	50	138
	9.0	27	26	64	56	107
	28.4	27	30	60	61	124
	90.0	28	32	61	65	123
	283.5	22	25	52	54	130
	900.0	26	23	56	54	112
Positive Control-1	3.15	-	-	245	308	387
	6.3	231	96	1298	-	1550
	10	519	58	-	-	1465
	20	890	-	-	-	-
Positive Control-2	10	21	150	599	781	520
Positive Control-3	90	22	85	800	676	3495
Positive Control-4	10	-	-	-	-	448
Vehicle Control		21	24	49	62	110

- Not done

Positive Control-1: 2-Aminoanthracene

Positive Control-2: Benzo(a)pyrene

Positive Control-3: 3-Methylcholanthrene

Positive Control-4: trans-N-Methyl-4-Aminostilbene

* 60 nl of epoxide hydratase inhibitor (1,1,1 Trichloro- propene-2,3-oxide) was added to each plate dissolved in 10 µl DMSO

Mean number of revertants without metabolic activation

Test substances	Dose / plate (µg)	TA1535	TA1537	TA98	TA100
Butyl acrylate	2.84	19	10	11	97
	9.0	13	14	16	149
	28.4	16	12	18	140
	90.0	20	8	11	130
	283.5	25	9	16	126
	900.0	21	11	15	135
Positive Control-1	1	903	7	19	232
	3.15	8500	31	29	4250
	10	31000	39	51	9250
Positive Control-2	2	17	572	3650	1060
Vehicle Control		19	8	15	117

- Not done

Positive Control-1: N-Methyl-N-Nitro-N-Nitrosoguanidine

Positive Control-2: Benzo(a)pyren-4-5-oxide

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Executive summary:

The results presented in this summary are experimental results for the source chemical n-butyl acrylate. The results are considered to be appropriate for read-across to the target chemical 2-methylbutyl acrylate.