1,1,1-trichloroethane

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Fairly recent work with clear objectives, description and results (not all results and no individual results presented). None guideline study and not GLP, but well designed to meet its objectives.

Data source

Reference

Reference Type:

publication

Title:

Acute, short-term, and subchronic oral toxicity of 1,1,1-trichloroethane in rats

Author:

BRUCKNER-JV; KYLE-GM; LUTHRA-R; ACOSTA-D; METHA-SM; SETHURAMAN-S; MURALIDHARA-S

Year:

2001

Bibliographic source:

TOXICOL-SCI 60 363-372

Materials and methods

Principles of method if other than guideline:

Study designed to assess the endpoint of liver toxicity and metabolism induction. Measurements made 24 hours after administration

GLP compliance:

no

Test type:

other: Single dose with 24 hours observation

Test material

Test material information

Constituent 1

Reference substance name:

1,1,1-trichloroethane

EC Number:

200-756-3

EC Name:

1,1,1-trichloroethane

Cas Number:

71-55-6

Molecular formula:

C2H3Cl3

IUPAC Name:

1,1,1-trichloroethane

Details on test material:

Analytical grade 99 %+ purity obtained from Aldrich Chemical Co. Purity confirmed by gas chromatography.

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Details on test animals or test system and environmental conditions:

Reverse light-dark conditions (light 2100 to 0900 hours), dose administered at start of active dark phase

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

Singel oral dose, total volume 1 ml

Doses:

0, 0.5, 1.0, 2.0 and 4 g/kg body weight

No. of animals per sex per dose:

Not specified, assumed 5

Control animals:

yes

Details on study design:

Euthansed after 24 hours and examination of blood and liver samples

Results and discussion

Mortality:

none

Clinical signs:

other: not reported

Gross pathology:

not reported

Other findings:

Histopathological examination and measurement of liver: body weight, serum enzymes, hepatic NPSH levels ad G-6-Phase activity did not reveal hepatocellular damage at any dosage level.

Applicant's summary and conclusion

Conclusions:

A single bolus administration of up to 4 g/kg body weight to the male rat was not associated with mortality or any indication of liver damage.

Executive summary:

In an acute study designed primarily to assess effects on the liver, a single bolus administration of up to 4 g/kg body weight to the male rat was not associated with mortality or any indication of liver damage. The animals were observed for 24 hours after administration.