6-methyloct-5-en-2-one

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17.09.2007 - 31.01.2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to OECD guideline under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

Not relevant

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

6-methyloct-5-en-2-one

EC Number:

246-073-4

EC Name:

6-methyloct-5-en-2-one

Cas Number:

24199-46-0

Molecular formula:

C9H16O

IUPAC Name:

6-methyloct-5-en-2-one

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

35 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 22 - 46

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

No other information

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-hour EC50 of Ethylheptenone was calculated to be 32 mg/L with 95% confidence limits of 22 - 46 mg/L. The 48-hour EC0 and the 48-hour NOEC (highest concentration tested without toxic effects after 48 hours) of Ethylheptenone were both 22 mg/L, since no immobilization was observed up to and including this test concentration. The 48 hour EC100 was 46 mg/L.

Executive summary:

The acute toxicity of the test item Ethylheptenone to Daphnia magna was determined in a 48-hour static test according to the EU Commission Directive 92/69/EEC, Part C.2 (1992) and the OECD Guideline for Testing of Chemicals, No. 202 (2004).

The nominal test item concentrations tested were 4.6, 10, 22, 46 and 100 mg/L. Additionally, a control group was tested in parallel.

Since Ethylheptenone was considered to be volatile, the test was performed in a closed system to avoid losses of the test item by evaporation.

At the start and the end of the test, the analytically determined concentrations of the test item in the analyzed test media were between 89 and 95% of the nominal values. This demonstrates the correct preparation of the test media and the stability of Ethylheptenone during the test period of 48 hours under the conditions of the test. Therefore, the reported biological results are based on the nominal concentrations of the test item.

24-hour EC50: 35 mg/L (95% confidence interval: could not be determined)

24-hour EC0: 22 mg/L

24-hour EC100: >100 mg/L

48-hour EC50: 32 mg/L (95% confidence interval: 22 - 46 mg/L)

48-hour EC0 and 48-hour NOEC: 22 mg/L

48-hour EC100: 46 mg/L