Pentasodium 4-amino-6-[[5-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-2-sulphonatophenyl]azo]-3-[(2,5-disulphonatophenyl)azo]-5-hydroxynaphthalene-2,7-disulphonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: screening tests

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: EEC Directive 84/449(1984), Part. C9

Deviations:

no

Principles of method if other than guideline:

A predetermined amount of the test substance dissolved in water was oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 h.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

None

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Details on inoculum:

None

Duration of test (contact time):

2 h

Initial conc.:

5 mg/L

Based on:

test mat.

Details on study design:

Sample weight: 5 mg of the test substance
Temperature: 148 ± 3 °C
Duration: 2 h
Estimation Technique: Titration (Potentiometric), METROHM Titroprozessor 670 (SAV : 00 111)

Reference substance:

other: Potassium hydrogen phthalate solution

Preliminary study:

No data

Test performance:

No data

Remarks on result:

not measured/tested

Details on results:

No data

Key result

Parameter:

COD

Value:

507 mg O2/g test mat.

Key result

Parameter:

BOD5

Value:

0 mg O2/g test mat.

Results with reference substance:

The COD of the reference substance is 204 mg O2/L (Criteria: 200±8 mg O2/L)

COD analytical data:

Product	Sample weight E (mg)	Titration (mL)			N=	mg O2/g
		a	b	c
Normality				10.108 10.116	0.1187 0.1186 Ǿ : 0.1186
Blank		9.818 9.804 Ǿ : 9.811
Test substance	5 mg 5 mg		7.146 7.130			506 509  Ǿ :507

Validity criteria fulfilled:

not applicable

Interpretation of results:

not inherently biodegradable

Conclusions:

The test substance showed no biodegradation potential.

Executive summary:

The chemical oxygen demand (COD) of the test substance was evaluated in this study.

A predetermined amount of the test substance (of 100 % purity) dissolved in water was oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 h. The residual dichromate was determined by titration with standardized ferrous ammonium sulphate (according to DEV H 41-1, DEV 1980).

The substance was tested at 5 mg/L.

Under the study conditions, the COD of the test substance was 507 mg O2/g. Further, the BOD5 value from another experiment was 0 mg O2/L. The % biodegradation was calculated using the formula: BOD/COD*100. Hence, the biodegradation of the test substance was 0 %.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Remarks:

BOD5

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 27 January, 1993 to 19 February, 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

ISO 5815 (Water quality - Determination of Biochemical Oxygen Demand after 5 Days (BOD5) - Dilution and Seeding Method)

Deviations:

yes

Remarks:

(aerate overnight after addition of the inoculum)

Principles of method if other than guideline:

No data

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

None

Oxygen conditions:

aerobic

Inoculum or test system:

other: Seeding water of an urban waste water

Details on inoculum:

- Source of inoculum/activated sludge: Seeding water of an urban waste water

Duration of test (contact time):

5 d

Initial conc.:

6.4 mg/L

Based on:

test mat.

Initial conc.:

12.8 mg/L

Based on:

test mat.

Initial conc.:

25.7 mg/L

Based on:

test mat.

Initial conc.:

51.4 mg/L

Based on:

test mat.

Initial conc.:

102.7 mg/L

Based on:

test mat.

Initial conc.:

205.5 mg/L

Based on:

test mat.

Initial conc.:

411 mg/L

Based on:

test mat.

Initial conc.:

821.9 mg/L

Based on:

test mat.

Details on study design:

Temperature: 20 ± 1 °C
Duration: 5 d
Estimation Technique: O2 determination, measured with an oxygen sensitive electrode system: SYLAND (SAV: 00 107)
Suppression of nitrification: without suppression of nitrification

Reference substance:

other: D(+)-Glucose /L-Glutamic acid

Preliminary study:

No data

Test performance:

No data

Remarks on result:

not measured/tested

Details on results:

The BOD5 of the test substance corrected by the blank control is 0 mg O2/g. Further, the COD value was taken from an another experiment and was found to be 507 mg O2/L. The % biodegradation was calculated using the below formula: BOD/COD*100.
Hence, the biodegradation of the test substance is 0 %.

Key result

Parameter:

BOD5

Value:

0 mg O2/g test mat.

Key result

Parameter:

COD

Value:

507 mg O2/g test mat.

Remarks on result:

other: from a different study

Results with reference substance:

The BOD5 of glucose/glutamic acid solution is 197 mg O2/L.

BOD values of the oxygen measurements:

Substance	Test concentration E = mg/L	Start mg O2/L T0	End mg O2/L T5	Blank mg O2/L B1	mg O2/g BOD5
Test substance	821.9	8.7 8.6	7.3 8.0	0.7 0.7	1 0
	411.0	8.7 8.6	8.0 7.9	0.7 0.7	0 0
	205.5	8.7 8.6	8.0 8.0	0.7 0.7	0 0
	102.7	8.7 8.6	8.2 8.0	0.7 0.7	0 0
	51.4	8.7 8.6	7.9 8.1	0.7 0.7	2 0
	25.7	8.7 8.6	8.0 8.2	0.7 0.7	0 0
	12.8	8.7 8.6	8.1 7.8	0.7 0.7	0 8
	6.4	8.7 8.6	8.1 8.1	0.7 0.7	0 0

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test substance showed no biodegradation potential.

Executive summary:

The biochemical oxygen demand of the test substance (of ca. 100 % purity) was determined by measuring the dissolved oxygen concentration before and after 5 d incubation at 20 °C with different test concentrations diluted in water saturated with dissolved oxygen and containing a seed of microorganisms. The test was carried out according to ISO 5815 in compliance with GLP.

The substance was tested at concentrations from about 6 to 800 mg/L.

Under the study conditions, the BOD5 of the test substance corrected by the blank control was 0 mg O2/g. Further, the COD value from another experiment was found to be 507 mg O2/L. The % biodegradation was calculated using the formula: BOD/COD*100. Hence, the biodegradation of the test substance was 0 %.

Reference 3

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 13 January, 1993 to 25 March, 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: ISO Method 8192 B

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

None

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: Sludge of a biological sewage treatment plant (ARA-Basel lndustrie)
- Amount of sludge: 910 mg/L

Duration of test (contact time):

28 d

Initial conc.:

150 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

Water/nutrient: According to the Guideline
Temperature: 22±3 °C (room temperature)
Test duration: 28 d
Lighting: Indirect daylight
Estimation technique: TOC/DOC analyzer / SHIMADZU TOC-500

Reference substance:

diethylene glycol

Preliminary study:

The mean value of a preliminary test was 154.5 mg/L.

Test performance:

No data

Key result

Parameter:

% degradation (DOC removal)

Value:

3.1

Sampling time:

28 d

Details on results:

The bioelimination of the test substance measured as DOC after 28 d corrected by the blank control was 0 % (average of 2 tests running in parallel). Further, the adsorption after 3 h was 0 %.

Results with reference substance:

The bioelimination, corrected by the blank control and measured as DOC (mg/L) was 99.1 % on the 23rd day.

Table:

Time	Reference			Test substance
	Mean value of the reference DOC (mg/L)	Value of the blank DOC (mg/L)	Elimination %	Mean value of the substance DOC (mg/L)	Value of the blank DOC (mg/L)	Elimination %
-1 d	153.5	-	-	154.5	-	-
0	169.8	19.6	2.2	172.5	19.6	1.0
3 h	170.1	14.3	0	175.7	175.7	0
2 d	169.1	13.4	0	179.2	13.4	0
5 d	175.0	21.7	0.1	194.6	21.7	0
7 d	165.6	20.4	5.4	191.6	20.4	0
9 d	153.3	18.7	12.3	187.4	18.7	0
12 d	153.3	18.7	12.3	187.4	18.7	0
13 d	147.4	19.8	16.9	190.1	19.8	0
14 d	67.1	14.7	65.9	180.8	14.7	0
15 d	19.0	12.4	95.7	178.8	12.4	0
16 d	17.4	11.7	96.3	-	-	-
19 d	13.7	11.6	98.6	182.4	11.6	0
21 d	12.8	9.3	97.7	-	-	-
23 d	11.9	10.5	99.1	170.8	10.5	0
26 d	-	-	-	179.0	12.4	0
28 d	-	-	-	171.9	9.8	0

Mean values of the double determination

 

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

The test substance is not inherently biodegradable.

Executive summary:

The inherent biodegradability of the test substance (of ca. 100 % purity) was determined in a 28 d Zahn-Wellens test according to OECD Guideline 302B and ISO Method 8192 B in compliance with GLP.

The substance was tested at a concentration of 150 mg/L DOC.

The bioelimination measured as DOC corrected by the blank control was 0 % (average of 2 tests running in parallel). Further, the adsorption after 3 h was 0 %.

Based on these findings, the test substance is not considered to be inherently biodegradable.

Description of key information

The biodegradability of Reactive Black 039 was determined based on the BOD5/COD quotient calculated. The test to determine the biological oxygen demand was performed on test concentrations from 6 to 800 mg/L following ISO 5815 (Water quality - Determination of Biochemical Oxygen Demand after 5 Days (BOD5) - Dilution and Seeding Method). In this study biodegradation of test substance was equivalent to 0 mg O2/g. The COD was estimated to be 507 mg O2/g in a separately conducted study. As a result the quotient of BOD5/COD is 0 indicating that the substance is not biodegradable.  In addition, an inherent biodegradation test following OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test) was conducted with the test item. As a result 3.1% DOC removal were found at the end of of the study period (44 days) supporting the initial finding, that the substance is neither readily nor inherently biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed