5-[[4-chloro-6-[(3-sulphophenyl)amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3-[[4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonic acid, sodium salt

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1999

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Not GLP study, very poor study.

Qualifier:

no guideline followed

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Parameter followed for biodegradation estimation:

DOC removal

Reference substance:

not specified

Parameter:

% degradation (DOC removal)

Value:

ca. 74

Parameter:

COD

Value:

ca. 554 mg O2/g test mat.

Parameter:

BOD5

Value:

ca. 410 mg O2/g test mat.

Validity criteria fulfilled:

not specified

Conclusions:

COD = 554 mg O2/g DYE BOD=410 mgO2/g DYE

Executive summary:

COD = 554 mg O2/g DYE BOD=410 mgO2/g DYE

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29. June to 24. August 1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

other: ISO 11734

GLP compliance:

not specified

Oxygen conditions:

anaerobic

Inoculum or test system:

anaerobic bacteria

Details on inoculum:

- Source of inoculum: Municipal sewage Kriftel
- Storage conditions: at 35°C +/- 2°C thermostat Incubator, the temperature has remained during the test in range of 33.0°C- 37.0°C
- Storage length: 7 days
- Preparation of inoculum for exposure: bacterial biomass anaerobic digester of a semi-continuous operation, through a 0.5 mm sieve to separate coarse particles, washed, resuspended to the DOC value of the class does not exceed 10 mg/L (C)
100 mL/L of anaerobic inoculum suspension with a solids content of 32.4 g/L, corresponding to 3.24 The final volume in the dry mass of the audit method.
- Pretreatment:the inoculum was 7 days for depletion of carbon in the incubator
mixed at 35 ± 2 °C
- Type and size of filter used, if any: 0.5 mm

Duration of test (contact time):

ca. 56 d

Initial conc.:

113 mg/L

Based on:

DOC

Initial conc.:

500 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Parameter followed for biodegradation estimation:

CH4 evolution

Details on study design:

TEST CONDITIONS
-Test vessel: 1000 mL glass tank with magnetic stirrer
- Composition of medium: ISO 11734 Punkt 6.2 Testmedium
- Additional substrate:
- Test temperature: 33 - 37 °C
- pH: 7.2
- pH adjusted: yes
- Suspended solids concentration: 100 mL/L
- Stock solution: 2000 mg/L; 2000 mg/800 mL weighed in highly purified water, measured pH 4.3; adjusted to pH 7.2, stirred for 2 hours at 22°C, measured pH 7.2, filled up to 1000 mL and used as stock solution.

TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
- Measuring equipment:
- Test performed in open system:
- Details of trap for CO2 and volatile organics if used: graduated gas mouse
- Other:

SAMPLING
- Sampling frequency:
- Sampling method:
- Sterility check if applicable:
- Sample storage before analysis:
- Other:

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control:
- Toxicity control:
- Other:

STATISTICAL METHODS:

Reference substance:

benzoic acid, sodium salt

Parameter:

% degradation (CO2 evolution)

Value:

< 20

Sampling time:

56 d

Parameter:

% degradation (CH4 evolution)

Value:

< 20

Sampling time:

56 d

Results with reference substance:

> 60% gas formation

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

A significant anaerobic biodegradation was not observed. The formation of gas within 56 days remained below 20% of the theoretical maximum.

Executive summary:

A significant anaerobic biodegradation was not observed. The formation of gas within 56 days remained below 20% of the theoretical maximum.

Description of key information

Not readily biodegradable

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Type of water:

freshwater

Additional information

The ready biodegradability of Reactive Red 198 was tested in anaerobic bacteria by ISO 11734 method. Methane and carbon dioxide released is determined quantitatively. From the amount of carbon released into the gas is formed in a ratio of carbon used to determine the contents of the rate of anaerobic biodegradation. This test is used for the examination for ultimate anaerobic biodegradability.  A significant anaerobic biodegradation was not observed. The formation of gas within 56 days remained below 20% of the theoretical maximum.