Acetic acid, 2-chloro-, reaction products with 2-C11-13-alkyl-4,5-dihydro-1H-imidazole-1-ethanol and sodium hydroxide

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

560 mg/L

Nominal / measured:

nominal

Conc. based on:

other: expressed as solid content

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

other: expressed as solid content

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Validity criteria fulfilled:

yes

Conclusions:

In accordance with OECD 209 (2010) and according to GLP principles the influence of a related member of the chemical category (amphoacetates C12) on the respiration rate of activated sludge was investigated after a contact time of 3 hours. The substance was not toxic to waste water (activated sludge) bacteria at a concentration of 560 mg/L (NOEC). This result is read-across to the registered substance.

Executive summary:

In accordance with OECD 209 the influence of a substance analogue (amphoacetates C12) on the respiration rate of activated sludge was investigated after a contact time of 3 hours. In a combined limit/range-finding test concentrations of 20, 200 and 2000 mg/l were tested. No statistically significant inhibition of the respiration rate of the sludge was recorded at the concentrations 20 and 200 mg/L. At 2000 mg/l, the average inhibition of the respiration was 70%. Therefore, a final test was performed at 100, 180, 320, 560 and 1000 mg/l. No statistically and biologically significant inhibition of the respiration rate of the sludge was recorded in this test at 560 mg/L (NOEC). At 1000 mg/L, the average inhibition of the respiration was 34%. The batch of activated sludge was checked for sensitivity by testing the reference substance 3,5-dichlorophenol, which showed normal sensitivity. The validity criteria were all fulfilled. The results are read-across to the registered substance.

Description of key information

In accordance with OECD 209 (2010) and according to GLP principles the influence of a substance analogue (amphoacetates C12) on the respiration rate of activated sludge was investigated after a contact time of 3 hours. The substance was not toxic to waste water (activated sludge) bacteria at a concentration of 560 mg/L (NOEC). This result is read-across to the registered substance.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

560 mg/L

Additional information

In accordance with OECD 209 the influence of substance analogue (amphoacetates C12) on the respiration rate of activated sludge was investigated after a contact time of 3 hours. In a combined limit/range-finding test concentrations of 20, 200 and 2000 mg/l were tested. No statistically significant inhibition of the respiration rate of the sludge was recorded at the concentrations 20 and 200 mg/L. At 2000 mg/l, the average inhibition of the respiration was 70%. Therefore, a final test was performed at 100, 180, 320, 560 and 1000 mg/l. No statistically and biologically significant inhibition of the respiration rate of the sludge was recorded in this test at 560 mg/L (NOEC). At 1000 mg/L, the average inhibition of the respiration was 34%. The batch of activated sludge was checked for sensitivity by testing the reference substance 3,5-dichlorophenol, which showed normal sensitivity. The validity criteria were all fulfilled. The results are read-across to the registered substance.