1-(N,N-bis(2-ethylhexyl)aminomethyl)-1,2,4-triazole

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November - December, 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

(1984)

Deviations:

yes

Remarks:

Instead of a centrifuged sludge a settled sludge was used. The substance was not dissolved but distributed in the test medium.

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 24.1, 6.4, 2.8, 1.1 and 0.38 mg of the test substance were weighed and added to the test medium. The volume was adjusted to 200 mL with water and aerated for 3 hours.

Test organisms (species):

activated sludge

Details on inoculum:

Activated sludge collected from the sewage treatment plant of CH - 4153 Reinach on 12/12/88. The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test temperature:

20 ± 2°C

Nominal and measured concentrations:

Nominal: 120.5, 32, 14, 5.5 and 1.9 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 ml BOD flasks with gas inlet
- Aeration: for 3 hours

TEST MEDIUM / WATER PARAMETERS
-Nutrient solution: in accordance to the guideline

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: Graphically determined

Results with reference substance (positive control):

LC50 = 14 mg/L (graphically determined)

Conclusions:

The EC50 (3h) of the substance EC 401-280-0 and its hydrolysate is 100mg/L

Description of key information

The EC50 (3h) of the substance EC 401-280-0 and its hydrolysate is 100mg/L

Key value for chemical safety assessment

EC50 for microorganisms:

100 mg/L

Additional information

Parent substance EC 401 -280 -0

The toxicity of the substance EC 401-280-0 to microorganisms was performed according to OECD-guideline no. 209 (1984). Mircoorganisms were exposed for three hours to the test item up to 120.5 mg/L. The EC50 was 100 mg/L (nominal).

hydrolysis products

bis(2-ethylhexyl)amine

In a supporting short-term respiration inhibition test according to OECD using activated sludge from a wwtp treating predominantly domestic sewage, the toxic effect of bis(2 -ethylhexyl)amine was studied. The 3 -h EC10 was determined to be 18 mg/L, while the 3 -h EC50 was 89 mg/L (BASF SE, 2012, report no. 08G0454/11G167).

In a short-term respiration inhibition test using activated sludge from a wwtp treating industrial wastewater, the EC20 of bis(ethylhexyl)amine was greater than 100 mg/L (BASF AG, 1983).

Additionally, in an oxygen consumption test usingPseudomonas putida(ROBRA test, Tween 80 was used as a solvent) an EC10 of 1300 mg/L for bis(2-ethylhexyl)amine was determined (BASF AG, 1989; report no. 10/1890500).

It can be concluded, that the inhibition of the degradation activity of activated sludge is not anticipated after adaptation.

1H-1,2,4 -triazole

No toxicity to microorganisms was observed (ECHA, 2014)

Formaldehyde

Formaldehyde is toxic to microorganisms (EC50 19mg/L) (Klecka 1985) which is to be expected as formaldehyde is used as disinfectant.