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EC number: 225-591-4 | CAS number: 4948-28-1
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26.09.2021 to 04.11.2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from aeration tank of sewage. Beijing, China.
- Concentration of sludge: 3 g suspended solids/L mineral medium
-Temperature: 21.2-22.7 °C - Duration of test (contact time):
- 28 d
- Initial conc.:
- 99.61 mg/L
- Based on:
- ThOD
- Initial conc.:
- 34.24 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Remarks:
- %ThOD
- Details on study design:
- TEST CONDITIONS
- Test temperature: 21.2-22.7 °C
- pH: 7.42-7.43
TEST SYSTEM
- Number of culture flasks/concentration: seven
- Measuring equipment: BOD meter OxiTop OC110 (WTW)
SAMPLING
- Sampling frequency: each 24 h
- Reference substance:
- other: Sodium benzoate C7H5NaO2
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 69.12
- Sampling time:
- 28 d
- Details on results:
- Degradation kinetics (%):
-Day 1: 14.16%
-Day 11: 60.74%
-Day 28: 69.12%
Toxicity control:
76.97% in 14 days (it is assumed that the test item is not inhibitory) - Results with reference substance:
- Biodegradation:
-Day 28: 81.94% - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The study showed that the test item is readily biodegradable. The test item fulfilled the 10-d window criterion (starting at day 1) and reached its pass level at the end of the 10-d window (day 11).
- Executive summary:
The present study was conducted in order to evaluate the biodegradability of the test item by measuring the percentage of biodegradation of inoculum to the test item within a period of 28 days. The test method was performed following the OECD Test Guideline 301 (F) for Manometric Respiratory Test, 1992 and GLP.
The test concentration was 32.24 mg test item/L (corresponding to ThOD NH3 of 99.61 mg O2/L). The 10-d window was fulfilled. After the 28-d period the biodegradation was calculated to be 69.12%. Therefore, the test item is regarded as readily biodegradable.
Reference
Description of key information
Readily biodegradable (69% after 28 days, OECD 301 F)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
The present study was conducted in order to evaluate the biodegradability of the test item by measuring the percentage of biodegradation of inoculum to the test item within a period of 28 days. The test method was performed following the OECD Test Guideline 301 (F) for Manometric Respiratory Test, 1992 and GLP.
The test concentration was 32.24 mg test item/L (corresponding to ThOD NH3 of 99.61 mg O2/L). The 10-d window was fulfilled. After the 28-d period the biodegradation was calculated to be 69.12%. Therefore, the test item is regarded as readily biodegradable.
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