Cuprate(4-), [C-(aminosulfonyl)-C-[[[2-[[4-chloro-6-[(2,5-disulfophenyl)amino]-1,3,5-triazin-2-yl]amino]ethyl]amino]sulfonyl]-29H,31H-phthalocyanine-C,C-disulfonato(6-)-N29,N30,N31,N32]-, sodium salts

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1 - 5 June 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

OECD Guidelines for Testing of Chemicals (1984) No. 203 "Fish, Acute Toxicity Test"

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

Method C.1 of Commission Directive 84/449/ EEC (which constitutes Annex V of Council Directive 67/ 548/ EEC) .

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

No further details specified in the study report.

Analytical monitoring:

yes

Details on sampling:

Ensured by daily renewal of the test media and verified by chemical analysis of the control and the 100 mg/ 1 test groups at 0, 24 and 96 hours.

Vehicle:

no

Details on test solutions:

Direct dispersion in water. 4.00 g of test material was dispersed in water with the aid of ultrasonic disruption and the volume adjusted to 2 litres to give a 4.00 g/l stock solution. 500 ml of this stock solution was dispersed in dechlorinated tap water and the volume adjusted to 20 litres to give a 100 mg/1 test concentration.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Name: Rainbow trout (Oncorhynchus mykiss).
Source: Parkwood Trout Farm, Wigmore, Kent, U.K.
Acclimatisation: Stock held since 13 May 1993 and acclimatised to test conditions from 25 May 1993.
The stock fish were maintained in a glass fibre tank with a “Single pass” water renewal system. The lighting was controlled to give a 16 hours light and 8 hours darkness cycle.
Temperature: 14.0 °C.
Dissolved Oxygen: ≥ 9.9 mg O2/l
Feeding: Commercial trout pellets daily. Discontinued 24h prior to test.
Mortalities: Zero in the 7 days prior to the start of the test.
Medication: None.
Mean standard length: 4.0 cm (SD = 0.2 cm).
Mean weight: 0.78 g (SD = 0.11 g).

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

No post exposure observation period specified in the study report.

Hardness:

approximately 50 mg/l as CaCo3.

Test temperature:

14.0 °C.

pH:

7.3 - 7.5

Dissolved oxygen:

9.9 - 10.0 mg O2/l

Salinity:

Not applicable-freshwater study

Conductivity:

Not specified

Nominal and measured concentrations:

Verification of test concentrations showed the measured test concentrations from analysis at 0, 24 and 96 hours to be near nominal. It was therefore considered justifiable to estimate the LC50 values in terms of nominal test concentrations only.

Details on test conditions:

Test vessels: Glass aquaria holding 20 1 of test media.
Experimental design: 1 test concentration in duplicate plus 1 control. 10 animals per replicate.
This experimental design conforms to a “Limit Test" to confirm that at the maximum test concentration given in the OECD/EEC test guidelines, no mortalities or adverse reactions to exposure were observed.
Method of initiation: Fish were placed, at random, in prepared test media.
Loading: 0.39 g bodyweight/ litre.
Photoperiod: 16 h light: 8 h dark.
Temperature: 14.0 °C.
Aeration: via narrow bore glass tubes.
Test concentration: 100 mg/l in duplicate. This test concentration was assigned following a preliminary range-finding study, where 3 fish were exposed to each of the following concentrations for a period of 96 hours: 1.0, 10 and 100 mg/l. The results showed no mortalities at all the test concentrations, thereby indicating the choice of test concentration employed.
Medium renewal: Daily
Duration of exposure: 96 hours.
Environmental Measurements: pH, dissolved oxygen concentration and temperature were recorded daily throughout the study.
Criteria of death: Absence of (i) respiratory movement and (ii) response to a physical stimulation.

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

There were no mortalities or other adverse reactions to exposure in 20 fish exposed to a test concentration of 100 mg/l for a period of 96 hours.

Inspection of the mortality data gave the following results based on nominal test concentrations:
Time (h) LC50 (mg/l)
3 >100
6 >100
24 >100
48 >100
72 >100
96 >100
Highest test concentration resulting in 0% mortality ≥ 100 mg/l
Lowest test concentration resulting in 100% mortality > 100 mg/l
"No Observed Effect Concentration" (NOEC) ≥ 100 mg/l

The "No Observed Effect Concentration" is based upon zero mortalities and the absence of any behavioural reactions to exposure at this concentration.
It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l.

Results with reference substance (positive control):

Not specified

Sublethal observations / clinical signs:

Cumulative Mortality Data for Rainbow Trout Exposed for 96 hours to JPR Blue 100

Nominal Concentration (mg/l)	Cumulative mortality (initial population: 10)
	3 h	6 h	24 h	48 h	72 h	96 h
Control	0	0	0	0	0	0
100      R1	0	0	0	0	0	0
100      R2	0	0	0	0	0	0

R₁– R₂= Replicates 1 and 2

 

Environmental Measurements for Rainbow Trout Exposed for 96 Hours to JPR Blue 100

Nominal Concentration (mg/l)	0 hours			24 hours			48 hours			72 hours			96 hours
	pH	mg O2/l	T °C	pH	mg O2/l	T °C	pH	mg O2/l	T °C	pH	mg O2/l	T °C	pH	mg O2/l	T °C
Control	7.3	9.9	14.0	7.4	9.9	14.0	7.5	10.0	14.0	7.4	10.0	14.0	7.4	10.0	14.0
100      R1	7.4	9.9	14.0	7.5	10.0	14.0	7.5	9.9	14.0	7.4	10.0	14.0	7.4	9.9	14.0
100      R2	7.4	9.9	14.0	7.5	10.0	14.0	7.5	9.9	14.0	7.5	9.9	14.0	7.4	10.0	14.0

R₁– R₂= Replicates 1 and 2

Validity criteria fulfilled:

not specified

Conclusions:

The following LC50 values are based on nominal test concentrations
Time (h) LC50 (mg/l)
3 >100
6 >100
24 >100
48 >100
72 >100
96 >100
"No Observed Effect Concentration" (NOEC) ≥ 100 mg/l

Executive summary:

TEST SPECIES: Rainbow trout (Oncorhynchus mykiss)

TEST TYPE: Acute toxicity. 96 h LC50

TEST CONDITIONS: Semi-static test conditions according to OECD Guideline No. 203 referenced as Method C.1 of Commission Directive 84/449/EEC (which constitutes Annex V of Council Directive 67/548/EEC).

 

The following LC50 values are based on nominal test concentrations

Time (h)        LC50 (mg/l)

3                    >100

6                    >100

24                  >100

48                  >100

72                  >100

96                  >100

"No Observed Effect Concentration" (NOEC)≥100 mg/l

 

It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l.

Description of key information

The following values are based on nominal test concentrations

96h LC50: >100 mg/l

"No Observed Effect Concentration" (NOEC )≥100 mg/l

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

TEST SPECIES: Rainbow trout (Oncorhynchus mykiss)

TEST TYPE: Acute toxicity. 96 h LC50

TEST CONDITIONS: Semi-static test conditions according to OECD Guideline No. 203 referenced as Method C.1 of Commission Directive 84/449/EEC (which constitutes Annex V of Council Directive 67/548/EEC) .

 

The following LC50 values are based on nominal test concentrations

Time (h)        LC50 (mg/l)

3                    >100

6                    >100

24                  >100

48                  >100

72                  >100

96                  >100

"No Observed Effect Concentration" (NOEC)≥100 mg/l

It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l.