Alfalfa, ext.

Partition coefficient

Link to relevant study record(s)

Reference

Endpoint:

partition coefficient

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 07 March to 23 August

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method)

Deviations:

yes

Remarks:

see Details on methods

Qualifier:

according to guideline

Guideline:

EU Method A.8 (Partition Coefficient - Shake Flask Method)

Deviations:

yes

Remarks:

see Details on methods

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Partition coefficient type:

octanol-water

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: AL01
- Expiration date of the lot/batch: 10.09.2019
- Purity test date: not available

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: refrigerated (2 to 8°C)
- Stability under test conditions: not available
- Solubility and stability of the test substance in the solvent/vehicle: not applicable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not applicable

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: none
- Preliminary purification step (if any): none
- Final dilution of a dissolved solid, stock liquid or gel: not applicable
- Final preparation of a solid: not applicable

OTHER SPECIFICS: none

Analytical method:

other: modified gravimetric

Key result

Type:

log Pow

Partition coefficient:

< -0.07

Temp.:

25 °C

Remarks on result:

other: pH not determined, see attached explanation

Test solutions concentrations and amounts

	Water				n-Octanol
Test solution	Initial weight (mg)	Final residue (mg)	Concen. (mg/L)	Number of mg in water	Residue (mg)	Concen. (mg/L)	Number of mg in octanol
1A	72.3298	38.02	760.40	54.75	17.58	244.18	17.58
1B	72.3298	38.63	772.60	55.63	16.70	231.98	16.70
2A	48.2198	15.83	211.07	20.26	27.96	582.45	27.96
2B	48.2198	22.96	306.13	29.39	18.83	392.31	18.83
3A	12.0550	1.56	41.60	2.00	N/A	N/A	N/A
3B	12.0550	5.07	135.20	6.49	5.57	57.97	5.57

N/A: Test 3A omitted from final calculation

Conclusions:

In the shake flask method conducted according to OECD test guideline 107 and to GLP, the LogPow value for Alfalfa, ext. was determined at < - 0.07 at 25 ± 0.5 °C.

Executive summary:

A shake flask test, conducted according to OECD test guideline 107 and to GLP, was performed to determine the Partition coefficient of Alfalfa, ext. The LogP_owvalue was determined at < - 0.07 at 25 ± 0.5°C.

Description of key information

LogP_ow: < - 0.07 at 25 ± 0.5°C.

Additional information

A shake flask test, conducted according to OECD test guideline 107 and to GLP, was performed to determine the Partition coefficient of Alfalfa, ext. The LogP_owvalue was determined at < - 0.07 at 25 ± 0.5°C.