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EC number: 268-130-2 | CAS number: 68003-46-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A defined amount of the test substance (stock solution = 2000 mg/L) was weighed into a measuring flask and the total volume was filled up with dilution water. The test bottles were conditioned about 15 min before the test was started.
- Differential loading: yes
- Controls: dilution water - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Length at study initiation: 2.0 cm (average length)
ACCLIMATION
- Acclimation period: at least 14 d
- Acclimation conditions: same as test
- Feeding frequency during acclimation: daily
FEEDING DURING TEST
- Frequency: no feeding - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 0 h: 18.7 °C (control), 18.7 - 18.8 °C (test solutions)
96 h: 18.8 °C (control), 18.5 - 18.8 °C (test solutions) - pH:
- 0 h: 7.5 (control), 7.6 - 7.7 (test solutions)
96 h: 7.5 (control), 7.7 - 7.9 (test solutions) - Dissolved oxygen:
- 0 h: 7.1 mg O2/L (control), 6.6 - 8.1 mg O2/L (test solutions)
96 h: 6.3 mg O2/L (control), 6.8 - 7.9 mg O2/L (test solutions) - Nominal and measured concentrations:
- nominal: control, 10, 25, 50, 100 and 200 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: beakers
- Fill volume: 7 L
- Aeration: yes, constant
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: chlorine-free drinking water; hardness: 37.4 mg/L Ca-content
- Metals: Copper content < 0.01 mg/L
- Chlorine: none
- Culture medium different from test medium: no
- Intervals of water quality measurement: The pH, O2 concentration and temperature were measured at 0, 24, 48, 72 and 96 h.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12 h light/dark cycle
- Light intensity: indirect day-similar light
EFFECT PARAMETERS MEASURED:
The fish were inspected after 24, 48, 72 and 96 h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - 2.5
- Range finding study: no - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 95.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: 0%
- Reported statistics and error estimates:
- The statistical evaluation is based on a Probit analysis. The calculation of the probit values with the date after 24 to 96 h was performed with the computer program "Dosis 50". For the experimental values of 0% and 100% no probit values are defined. For the calculation of the probit values of these percentages the percentages 1% and 99% were used.
- Sublethal observations / clinical signs:
The highest tested concentration of test substance producing no mortality under test conditions after 96 h was 50 mg/L.
The lowest tested concentration of test substance producing 100% mortality under test conditions after 96 h was 200 mg/L.
Table 1. Mortality in 24 h intervals.
Concentration of test substance [mg/L]
Mortality [%]
After 24 h
Mortality [%]
After 48 h
Mortality [%]
After 72 h
Mortality [%]
After 96 h
200
100
100
100
100
100
14
57
57
57
50
0
0
0
0
25
0
0
0
0
10
0
0
0
0
control
0
0
0
0
- Validity criteria fulfilled:
- yes
Reference
Description of key information
LC50 (96 h) = 95.8 mg/L (nominal, OECD 203, Danio rerio)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 95.8 mg/L
Additional information
One GLP-study according to OECD 203 investigating the short-term toxicity of Ammonium N-methyl-N-(1-oxododecyl)glycinate (CAS 68003-46-3) to freshwater fish is available. The test organism Danio rerio was exposed for 96 h to five nominal test concentrations (10, 25, 50, 100 and 200 mg/L). No analytical monitoring of test concentrations was performed. Mortality was recorded daily and was compared to the negative control. After 96 h mortality was observed at concentrations ≥ 100 mg/L (nominal). The derived nominal LC50 value after 96 h was 95.8 mg/L (nominal).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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