Ammonium 2-(acryloylamino)-2-methylpropane-1-sulfonate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study conducted to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-acrylamido-2-methylpropanesulfonic acid

IUPAC Name:

2-acrylamido-2-methylpropanesulfonic acid

Constituent 2

Reference substance name:

ammonium (1Z)-N-(2-methyl-1-sulfopropan-2-yl)prop-2-enimidate

EC Number:

611-646-3

Cas Number:

58374-69-9

Molecular formula:

C7H12NO4S.H4N

IUPAC Name:

ammonium (1Z)-N-(2-methyl-1-sulfopropan-2-yl)prop-2-enimidate

Details on test material:

- Name of test material (as cited in study report): OS 114454
- Substance type: organic
- Physical state: very pale brown clear liquid
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: 50%
- Purity test date: no data
- Lot/batch No.: no data
- Expiration date of the lot/batch: no data
- Stability under test conditions: stable
- Storage condition of test material: oom temperature

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 200 mg/l
- Sampling method: no data
- Sample storage conditions before analysis: no data

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: Fathead minnow
- Strain:
- Source: Neil Hardy Aquatica Carshalton, UK,
- Age at study initiation (mean and range, SD): juvenile
- Length at study initiation: 3.8 cm (SD 0.4)
- Weight at study initiation: 0.54 g (SD 0.12)
- Feeding during test: no
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test
- Type and amount of food: Tetramin flake food
- Feeding frequency: no data
- Health during acclimation: 3% mortality in 7 days prior to test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

No

Test conditions

Hardness:

100 mgll as CaC03

Test temperature:

21±1°C

Dissolved oxygen:

>7.6 mg O2/l

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal: 200, 360, 640, 1120 and 2000 mg/l
Measured: 200, 367, 648, 1133 and 2066 mg/l at study initiation

Details on test conditions:

TEST SYSTEM
- Test vessel: glass
- Type: closed
- Fill volume: 20 l
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechloinated laboratory tap water
- Total organic carbon: 1 mg/l
- Particulate matter: no data
- Metals: all below the prescribed concentration
- Pesticides: <0.08 µg/l (all below the prescribed concentration)
- Chlorine: 0.22 mg/l
- Alkalinity: 80 mg/l
- Ca/mg ratio: 5:1
- Conductivity: 382 MICSM
- Intervals of water quality measurement: initially and every 24 hours for temerature, pH and dissolved oxygen
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hours light/8 hours darkness
- Light intensity: no data
EFFECT PARAMETERS MEASURED: mortality and behavioural effects
TEST CONCENTRATIONS
- Spacing factor for test concentrations: no data
- Justification for using less concentrations than requested by guideline: based on range-finding study
- Range finding study
- Test concentrations: 200 and 2000 mg/l
- Results used to determine the conditions for the definitive study: yes

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

640 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

other: mortality and behaviour

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1 400 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 1300-1600 mg/l

Details on results:

- Behavioural abnormalities: yes, loss of equilibrium at concentrations greater than 640 mg/l
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: none

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute LC50 at 96 hours exposure of the test article to the freshwater fish Fathead Minnow (Pimephales promelas) was determined to be1400 mg/l with 95% confidence limits of 1300-1600 mg/l. The No Observed Effect Concentration (NOEC) was 640 mg/l.