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EC number: 278-189-6 | CAS number: 75314-27-1
Endpoint summary
Administrative data
Description of key information
Short-term toxicity to aquatic invertebrates:
In a prediction done by SSS (2018) using the OECD QSAR toolbox with log kow as the primary descriptor, the short term aquatic toxicity to invertebrates was estimated forDisodium [6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]-N-methylnaphthalene-2-sulphonamidato(2-)] [6-amino-5- [(2-hydroxy-4-nitrophenyl) azo]naphthalene-2-sulphonato(3-)]cobaltate(2-). The EC50 was estimated to be 15.14 mg/l when Daphnia magna was exposed withDisodium [6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]-N-methylnaphthalene-2-sulphonamidato(2-)] [6-amino-5- [(2-hydroxy-4-nitrophenyl) azo]naphthalene-2-sulphonato(3-)]cobaltate(2-).
On the basis of the effect concentration the test chemical is considered toxic and can be classified in the category "Aquatic chronic 3" as per CLP classification.
Toxicity to aquatic algae and cynobacteria:
In a prediction done by SSS (2018) using the OECD QSAR toolbox with log kow as the primary descriptor, the short term aquatic toxicity to algae and cynobacteria was estimated forDisodium [6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]-N-methylnaphthalene-2-sulphonamidato(2-)] [6-amino-5- [(2-hydroxy-4-nitrophenyl) azo]naphthalene-2-sulphonato(3-)]cobaltate(2-). The EC50 was estimated to be 32.6 mg/l when Daphnia magna was exposed withDisodium [6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]-N-methylnaphthalene-2-sulphonamidato(2-)] [6-amino-5- [(2-hydroxy-4-nitrophenyl) azo]naphthalene-2-sulphonato(3-)]cobaltate(2-).
On the basis of the effect concentration the test chemical is considered toxic and can be classified in the category "Aquatic chronic 3" as per CLP classification.
Additional information
Short-term toxicity to aquatic invertebrates:
In a prediction done by SSS (2018) using the OECD QSAR toolbox with log kow as the primary descriptor, the short term aquatic toxicity to invertebrates was estimated forDisodium [6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]-N-methylnaphthalene-2-sulphonamidato(2-)] [6-amino-5- [(2-hydroxy-4-nitrophenyl) azo]naphthalene-2-sulphonato(3-)]cobaltate(2-). The EC50 was estimated to be 15.14 mg/l when Daphnia magna was exposed withDisodium [6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]-N-methylnaphthalene-2-sulphonamidato(2-)] [6-amino-5- [(2-hydroxy-4-nitrophenyl) azo]naphthalene-2-sulphonato(3-)]cobaltate(2-).
On the basis of the effect concentration the test chemical is considered toxic and can be classified in the category "Aquatic chronic 3" as per CLP classification.
The above prediction for target was suppported by experimental result for structurally similar read across substance sodium 4-[(2-hydroxy-1-naphthyl) diazenyl]benzenesulfonate (CAS: 633 -96 -5) summarized in Journal of Hazardous Materials, Vol. 276, Pg. no. 332–338, 2014.
Short term toxicity study toDaphnia similiswas carried out for 48 hrs. Neonates of less than 24 h old isolated from Daphnia culture were used for the study. Five test organisms were placed in each screw cap tubes, filled with 10 mL solution, and then incubated at 21±0.3°C in the dark. After 48 h of exposure, the number of immobile organisms was recorded. Jspear software was used to calculate the median 50% immobilization concentration (EC50). Based on immobilization of test organism by test chemical Acid Orange 7, the EC50 value was determine to be 87 mg/l along with confidence interval (p – 0.05) of 76-99 mg/l.
The above experiental data for read across substance was further supported by another experimental study for structurally similar read across substanceDisodium 8-(phenylamino)-5-[[4-[(5 sulphonatonaphthyl) azo]naphthyl] azo]naphthalene sulphonate(CAS: 3071 -73 -6).
Disodium 8-(phenylamino)-5-[[4-[(5 sulphonatonaphthyl) azo]naphthyl] azo]naphthalene sulphonate was used to evaluate short term toxicity on 24 h old neonates Daphnia magna as per OECD guideline 202. Untreated ISO formulation freshwater and potassium dichromate (0.32 – 3.2 mg/L) were used as negative and positive controls respectively.The effect concentration (LC50) with Daphnia magna at 48 h was observed to be 58.22 mg/l.
Toxicity to aquatic algae and cynobacteria:
In a prediction done by SSS (2018) using the OECD QSAR toolbox with log kow as the primary descriptor, the short term aquatic toxicity to algae and cynobacteria was estimated forDisodium [6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]-N-methylnaphthalene-2-sulphonamidato(2-)] [6-amino-5- [(2-hydroxy-4-nitrophenyl) azo]naphthalene-2-sulphonato(3-)]cobaltate(2-). The EC50 was estimated to be 32.6 mg/l when Daphnia magna was exposed withDisodium [6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]-N-methylnaphthalene-2-sulphonamidato(2-)] [6-amino-5- [(2-hydroxy-4-nitrophenyl) azo]naphthalene-2-sulphonato(3-)]cobaltate(2-).
On the basis of the effect concentration the test chemical is considered toxic and can be classified in the category "Aquatic chronic 3" as per CLP classification.
The above prediction was supported experimental study conducted by Sustainability Support Services (Europe) AB for the functionally similar read across substance Tetrasodium 1-acetamido-2-hydroxy-3-(4-((4-sulphonatophenylazo)-7-sulphonato-1-naphthylazo))naphthalene-4,6-disulphonate (2519 -30 -4).
Aim of this study was to assess the effect of test item tetrasodium 1-acetamido-2-hydroxy-3-(4-((4-sulphonatophenylazo)-7-sulphonato-1-naphthylazo))naphthalene-4,6-disulphonate, CAS No. 2519-30-4 on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test.
The chemical was analitycally evaluated by using spectrophotometer, the absorbance values of each test vessel and control vessel was noted at 680nm.The BBM was taken as blank for both control and test vessels. The absorbance value of each vessel was in line with the average specific growth rate. The test solution was prepared in aseptic condition. The test item tetrasodium 1-acetamido-2-hydroxy-3-(4-((4-sulphonatophenylazo)-7-sulphonato-1-naphthylazo))naphthalene-4,6-disulphonate was prepared by adding 21 mg of test item in 200ml of BBM to get the final concentration of 105 mg/L. This stock solution was kept for stirring for 30 minutes to obtain a homogenous solution for the experiment. The concentrations were chosen according to the available data of the test item. The remaining test solutions were prepared by dilution from the above stock solution. To have a better growth and visibility of cells, the initial cell density of the culture was kept 1 X 104cells/ml. Care was taken to have a homogeneous solution for the experiment.
Test organism green alga Chlorella vulgaris were collected fom National Environmental Engineering Research Institute (NEERI), Nagpur and cultivate in Bold’s Basal Medium (BBM). For the assessment of algal growth, the study was conducted in replicates. The control vessel was maintained in triplicates as recommended in the OECD guideline and the test concentrations were selected in geometric series which were maintained in duplicates.
After the exposure of chemical effect were measured. To obtain a quantitative concentration-response relationship by regression analysis, a linearizing transformation of the response data into probit was performed. Using the same, effective concentration (EC) was determined. The microscopic observations were also noted in each of the test vessel. All the cells appeared healthy, round and green throughout the study duration in the control and in the test vessels cells appeared shrinked and black dotted rings observed in concentrations of 0.96mg/l, 3.072mg/l, 9.830mg/l, 31.457mg/l & 100.663mg/l.
After 72 hours of exposure to test item tetrasodium 1-acetamido-2-hydroxy-3-(4-((4-sulphonatophenylazo)-7-sulphonato-1-naphthylazo))naphthalene-4,6-disulphonate (CAS No. 2519-30-4) to various nominal test concentrations, EC50 was determine to be 24.342 mg/l graphically and through probit analysis. Thus based on the EC50, chemical was concluded as toxic and can be consider to be classified as aquatic chronic 3 category as per the CLP classification criteria.
The above experimental data was further supported by experimental data for another functionally similar read across substance Disodium 8-(phenylamino)-5-[[4-[(5 sulphonatonaphthyl) azo]naphthyl] azo]naphthalene sulphonate (3071-73-6).
he 72 h algal growth inhibition test with Raphidocelis subcapitata was done according to OECD 201. Algae were routinely cultured at 25±1 °C with light in Cyanobacteria BG-11 Freshwater Solution. Algae were exposed to the treatment in sterile 24-well plates with 2 mL of test material.
The effect concentration (EC50) for the test material against Raphidocelis subcapitata was observed to be 67.2 mg/l
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