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EC number: 946-410-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02 March 2015 to 13 March 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Radiolabelling:
- no
- Test temperature:
- 30 °C
- Details on study design: HPLC method:
- EQUIPMENT
- Test utilized a high performance liquid chromatograph. A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.
DETERMINATION OF DEAD TIME
- Method: The dead time was determined by measuring the retention time of formamide (purity: 99.94%) at 622 mg/L in methanol:water (55:45 v/v).
REFERENCE SUBSTANCES
- Solutions of reference standards (table 5.4.1/1) were prepared in methanol.
PREPARATION OF THE SAMPLE SOLUTION
- Test item (0.1038 g) was diluted to 10 mL with methanol.
DETERMINATION OF RETENTION TIMES
- The sample, dead time and reference standard solutions were injected in duplicate using the following high performance liquid chromatography (HPLC) parameters:
HPLC system : Agilent Technologies 1200, incorporating workstation and autosampler
Detectors: Agilent variable wavelength detector (VWD) and Polymer Laboratories evaporative light scattering detector 2100 (ELSD)
Column : Water XSelect CN 5 µm (150 x 4.6 mm id)
Column Temperature : 30 °C
Flow rate : 1.0 mL/min
Mobil phase : methanol: purified water (55:45 v/v)
pH of mobile phase : 6.5
Injection volume : 10 μL
UV detector wavelength (dead time and reference standards): 210 nm
ELSD parameters (sample): nebuliser temperature: 40 °C; evaporator temperature: 80 °C; gas flow: 1.0 L/min
The mobile phase was ramped to 100% propan-2-ol shortly after the elution of the last reference standard to elute the highly retained test item components. This was carried out for the sample and sample blank injections only.
EVALUATION
- Calculation of capacity factors k':
The capacity factors were determined using the following equation:
k’ = (tr– t0)/ t0
where:
k’: capacity factor; tr: retention time (min); t0: dead time (min)
Construction of the calibration curve:
A correlation of logo k’ versus Log10Koc of the calibration standards was plotted using linear regression. The capacity factor (k’) for the reference standards was calculated from the retention time data of the dead time and reference standard solutions. Log10Koc values of the reference standards are those quoted in OECD 121.
- Determination of the log Koc value:
Adsorption Coefficient:
Log10Koc = (Log10k’-A) /B
Koc: adsorption coefficient
k’: capacity factor
A: intercept of the calibration curve
B: slope of the calibration curve - Type:
- Koc
- Remarks on result:
- other: >4.27 x 10E05
- Type:
- log Koc
- Value:
- > 5.63
- Temp.:
- 30 °C
- Details on results (HPLC method):
- - Typical chromatograms are attached.
- Retention times of the dead time and the retention times, capacity factors and log10 Koc values for the reference standards are shown in Tables 3.2 and 3.3 (below).
- The calibration curve is shown in Figure 3.1 (attached).
- The retention times, capacity factor and log10 Koc value determined for the sample are shown in Table 3.4 (below). - Validity criteria fulfilled:
- yes
- Conclusions:
- The adsorption coefficient of the test item was assessed in accordance with OECD Guideline 121 and has been determined to be greater than 4.27 x 10E05 with log10 Koc > 5.63.
- Executive summary:
GUIDELINE
The determination was carried out using the HPLC screening method, designed to be compatible with Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001, and Method C.19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008.
METHOD
The test utilized a high performance liquid chromatograph. A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.
RESULTS
The adsorption coefficient of the test item has been determined to be greater than 4.27 x 10E05 with log10 Koc > 5.63.
- Endpoint:
- adsorption / desorption: screening
- Remarks:
- Further information on adsorption/ desorption
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- the study does not need to be conducted because the substance and its relevant degradation products decompose rapidly
Referenceopen allclose all
Table 3.2: Retention times of the dead time
Dead Time |
Retention Time (min) |
Mean Retention Time (min) |
|
Injection 1 |
Injection 2 |
||
Formamide |
1.919 |
1.921 |
1.920 |
Table 3.3: Retention times, capacity factors andLog10 Koc values for the reference standards
Standard |
Retention Time (min) |
Mean Retention Time (min) |
Capacity Factor (k’) |
Log10k’ |
Log10Koc |
|
Injection 1 |
Injection 2 |
|||||
Acetanilide |
2.625 |
2.625 |
2.625 |
0.367 |
-0.435 |
1.25 |
Atrazine |
3.615 |
3.600 |
3.607 |
0.879 |
-5.62 x 10-2 |
1.81 |
Isoproturon |
3.840 |
3.833 |
3.837 |
0.998 |
-8.24 x 10-4 |
1.86 |
Triadimenol |
5.129 |
5.126 |
5.127 |
1.67 |
0.223 |
2.40 |
Linuron |
5.213 |
5.209 |
5.211 |
1.71 |
0.234 |
2.59 |
Naphthalene |
4.534 |
4.532 |
4.533 |
1.36 |
0.134 |
2.75 |
Endosulfan-diol |
6.376 |
6.378 |
6.377 |
2.32 |
0.366 |
3.02 |
Fenthion |
7.647 |
7.649 |
7.648 |
2.98 |
0.475 |
3.31 |
α-Endosulfan |
10.762 |
10.752 |
10.757 |
4.60 |
0.663 |
4.09 |
Diclofop-methyl |
11.584 |
11.589 |
11.587 |
5.03 |
0.702 |
4.20 |
Phenanthrene |
8.338 |
8.333 |
8.336 |
3.34 |
0.524 |
4.09 |
DDT |
22.630 |
22.632 |
22.631 |
10.8 |
1.03 |
5.63 |
Table 3.4: Adsorption coefficient of sample
Injection |
Retention Time (min) |
Capacity Factor (k’) |
Log10k’ |
Log10Koc |
Mean Log10Koc |
Adsorption Coefficient |
1 |
≥23.223 |
>10.8 |
>1.03 |
>5.63 |
>5.63 |
>4.27 x 105 |
2 |
≥23.333 |
>10.8 |
>1.03 |
>5.63 |
Description of key information
The adsorption coefficient of the test item was assessed in accordance with OECD Guideline 121 and has been determined to be greater than 4.27 x 10E05 with log10 Koc > 5.63.
Key value for chemical safety assessment
Additional information
Adsorption / desorption
GUIDELINE
The determination was carried out using the HPLC screening method, designed to be compatible with Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001, and Method C.19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008.
METHOD
The test utilized a high performance liquid chromatograph. A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.
RESULTS
The adsorption coefficient of the test item has been determined to be greater than 4.27 x 10E05 with log10 Koc > 5.63. Further information on adsorption / desorptionThe test item is not classified as hazardous to the environment and has been demonstrated to be readily biodegradable. As a result, and in accordance with REACH Annex IX, Section 9.3.3, Column 2, further information on adsorption/desorption is not considered relevant or necessary.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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