Bis(2-hydroxyethyl) sulphone

Hydrolysis

Link to relevant study record(s)

Reference

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Study period:

The study was conducted between 6 March 1998 and 17 May 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 111 (Hydrolysis as a Function of pH)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Sponsor's identification: BHES-100X
Batch number: 1A0110
Date received: 20 January 1998
Storage conditions: room temperature in the dark

Radiolabelling:

no

Analytical monitoring:

yes

Details on sampling:

Aliquots of the sample solutions were taken from the flasks at various times and the pH of each solution recorded.
The concentration of the sample solution was determined by HPLC.
Duplicate aliquots (A and 8) of sample solutions were diluted by a factor of two using acetonitrile.
Duplicate standard solutions of test material were prepared in acetonitrile:buffer (1: 1 v/v) at a nominal concentration of 500 mg/I.

Buffers:

See table in 'any other information on metrials and methods' section

Details on test conditions:

Preparation of samples
Sample solutions were prepared in stoppered glass flasks at a nominal concentration
of 1 g/I in the three buffer solutions.
The solutions were shielded from light.

Preliminary test
Sample solutions at pH 4, 7 and 9 were maintained at 50.0 ± 0.5 °C for a period
of 120 hours.

Duration:

120 h

pH:

4

Temp.:

50 °C

Initial conc. measured:

1 g/L

Duration:

120 h

pH:

7

Temp.:

50 °C

Initial conc. measured:

1 g/L

Duration:

120 h

pH:

9

Temp.:

50 °C

Initial conc. measured:

1 g/L

Positive controls:

no

Negative controls:

no

Preliminary study:

After incubation at 50 °C for 120 hrs > 96% BHES-100X was recovered at pH 4, 7 and 9.

Transformation products:

not measured

% Recovery:

101

pH:

4

Temp.:

50 °C

Duration:

120 h

% Recovery:

97.5

pH:

7

Temp.:

50 °C

Duration:

120 h

% Recovery:

96.9

pH:

9

Temp.:

50 °C

Duration:

120 h

pH:

4

Temp.:

25 °C

DT50:

> 1 yr

Remarks on result:

hydrolytically stable based on preliminary test

pH:

7

Temp.:

25 °C

DT50:

> 1 yr

Remarks on result:

hydrolytically stable based on preliminary test

pH:

9

Temp.:

25 °C

DT50:

> 1 yr

Remarks on result:

hydrolytically stable based on preliminary test

The following tables show the test material concentrations at the given timepoints.

pH4 at 50 ± 0.5 °C

Time (hours)	0	2.4	120
Concentration (g/l)	1.01	1.01	1.02
% of initial	100	99.9	101

Result: Less than 10% hydrolysis after 5 days at 50°C, equivalent to a half-life greater than 1 year at 25°C.

pH7 at 50 ± 0.5°C

Time (hours)	0	2.4	120
Concentration (g/l)	1.07	1.04	1.04
% of initial	100	97.6	97.5

Result: Less than 10% hydrolysis after 5 days at 50°C, equivalent to a half-life greater than 1 year at 25°C.

pH9 at 50 ± 0.5°C

Time (hours)	0	2.4	120
Concentration (g/l)	1.03	1.02	0.999
% of initial	100	98.9	96.9

Result: Less than 10% hydrolysis after 5 days at 50°C, equivalent to a half-life greater than 1 year at 25°C.

Validity criteria fulfilled:

not specified

Conclusions:

The estimated half-lives at 25 °C of BHES-100X are greater than 1 year a pH 4, 7 and 9.

Executive summary:

Assessment of hydrolytic stability was carried out using Method 111 of the OECD Guidelines for the Testing of Chemicals, 12 May 1981. The results are shown in the table below.

pH	Estimated half-life at 25°C
4	Greater than 1 year
7	Greater than 1 year
9	Greater than 1 year

Description of key information

The estimated half-lives at 25 °C of BHES-100X are greater than 1 year a pH 4, 7 and 9.

Key value for chemical safety assessment

Half-life for hydrolysis:

1 yr

at the temperature of:

25 °C