Registration Dossier
Registration Dossier
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EC number: 202-525-2 | CAS number: 96-69-5
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The one available study for this endpoint is a prolonged acute toxicity test determining LC50 values over a 14-d period with fathead minnows (Pimephales promelas). The study was not performed under GLP but followed the guideline of US-EPA Ecol. Res. Ser. 660/3-750 (Methods of acute toxicity tests with fish, macroinvertebrates and amphibians). LC50 values and 95 % confidence intervals were determined after 24h, 48h, 72, 96h, 7d and 14d, and were based on measured concentrations. The key value for this study was the 96-h LC50 = 0.14 mg/L. This value was used to derive the PNECs aquatic.
Key value for chemical safety assessment
Additional information
The results of this prolonged toxicity test are not appropriate to derive the required key value for long-term toxicity. Although the study is non-GLP and did not follow currently valid and/or internationally excepted test guidelines, it is found reliable. Therefore, the 96-h LC50 value could be applied to derive aquatic PNEC values. The study illustrated the time dependence of LC50 values in fathead minnows exposed in a flow-through system. After 24 hours, an LC50 of 0.21 mg/L was observed. This level gradually decreased over time to 0.17 mg/L after 48 hours, 0.15 mg/L after 72 hours, 0.14 mg/L after 96 hours, 0.08 mg/L after 7 days and 0.054 mg/L after 14 days.
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