Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-729-0 | CAS number: 1369500-14-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2011-04-19 to 2011-05-12 (experimental phase)
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Version / remarks:
- adopted February 24, 1987
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.3 (Acute Toxicity (Dermal))
- Version / remarks:
- from May 30, 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1200 (Acute Dermal Toxicity)
- Version / remarks:
- from August 1998
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- (1R,4S,4aR,8aS)-9-(dichloromethylidene)octahydro-1,4-methanonaphthalene-5,8-dione
- EC Number:
- 700-729-0
- Cas Number:
- 1369500-14-0
- Molecular formula:
- C12H12Cl2O2
- IUPAC Name:
- (1R,4S,4aR,8aS)-9-(dichloromethylidene)octahydro-1,4-methanonaphthalene-5,8-dione
- Details on test material:
- - Physical state: Brown powder
- Stability under test conditions: not reported
- Storage condition of test material: At room temperature (20 ± 5 °C), light protected
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: RccHan: WIST(SPF)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: males: 9 weeks, females: 11 weeks
- Weight at study initiation: males: 250.4 g - 260.8 g, females: 190.8 g - 208.2 g
- Fasting period before study: No
- Housing: During acclimatization in groups of five per sex in Makrolon type-4 cages with standard softwood bedding, including paper enrichment. Individually in Makrolon type-3 cages and under similar conditions during treatment and observation.
- Diet (e.g. ad libitum): Pelleted Teklad Rat-Mouse Diet 2914C ad libitum
- Water (e.g. ad libitum): Tap water ad libitum
- Acclimation period: At least 5 days, under laboratory conditions and after health examination. Only animals without any visible signs of illness were used for the study.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 3
- Humidity (%): 30 - 70
- Air changes (per hr): 10-15
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: To:
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- water
- Details on dermal exposure:
- TEST SITE
- Area of exposure: backs of the animals
- Type of wrap if used: The patch was covered with a semi-occlusive dressing which was wrapped around the abdomen and fixed with an elastic adhesive bandage.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): washed with lukewarm tap water and dried with disposable paper towels
- Time after start of exposure: 24 hours
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2000 mg/kg body weight
- Constant volume or concentration used: not applicable (only 1 concentration)
- For solids, paste formed: yes
VEHICLE
- Amount(s) applied (volume or weight with unit): The test item was moistened with a minimum of purified water to obtain a dry paste in order to guarantee good skin contact. - Duration of exposure:
- 24 hours
- Doses:
- 2000 mg/kg body weight (applied by weight as supplied by the Sponsor)
- No. of animals per sex per dose:
- group 1: 1 male, 1 female (Animal no. 1 and 2, treated 2 days before the other rats)
group 2: 4 males, 4 females - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: The animals were examined daily during acclimatization (mortality and clinical signs). All animals were examined for clinical signs before treatment, once within the first 30 minutes and approximately 1, 2, 3 and 5 hours after treatment on test day 1 and once daily during test days 2 to 15. Local dermal signs were assessed once daily from test day 2 to 15. Mortality was assessed before treatment, once within the first 30 minutes and approximately 1, 2, 3 and 5 hours after treatment on test day 1 (with the clinical signs) and twice daily during days 2 to15. Body weights were recorded on test day 1 (prior to administration) and on test days 8 and 15. All animals were examined macroscopically after being sacrificed at the end of the study.
- Necropsy of survivors performed: yes
- Other examinations performed: mortality, clinical signs, local dermal signs, body weight, macroscopy
Results and discussion
- Preliminary study:
- 2 rats (1 male (animal no. 1), 1 female (animal no. 2)) were treated 2 days before the other rats. The findings for these were:
- no deaths, no clinical signs, no macroscopic findings
- body weights within commonly recorded range
- focal crusts after test item application lasting for 13 days in male rat
- Brown discoloration of the skin in male and female rat lasting up to the end of the observation period
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No deaths occurred during the course of the study.
- Clinical signs:
- No clinical signs were observed during the course of the study.
- Body weight:
- One female slightly lost body weight in the second week after treatment (2%). The body weights of the other animals were within the range commonly recorded for this strain and age.
- Gross pathology:
- No macroscopic findings were recorded at necropsy.
- Other findings:
- Local dermal signs: Four males and one female showed focal crusts after test item application lasting for a minimum of 2 and a maximum of 13 days. Brown discoloration of the skin was observed in all animals for a minimum of 7 days after test item application and lasted up to the end of the observation period in 3 males and 3 females.
Any other information on results incl. tables
Local Dermal Signs
Animal no/sex |
Dose [mg/kg] |
Local dermal signs (max grade) |
Test day |
|||||||||||||
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
|||
1 / M |
2000 |
Focal crusts |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
. |
Brown discolouration |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
1 |
1 |
1 |
1 |
1 |
||
2 / F |
2000 |
Brown discolouration |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
3/ M |
2000 |
Focal crusts |
. |
. |
. |
. |
. |
1 |
1 |
1 |
1 |
1 |
. |
. |
. |
. |
Brown discolouration |
2 |
2 |
2 |
2 |
2 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
||
4 / M |
2000 |
Focal crusts |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
. |
. |
. |
. |
. |
. |
. |
Brown discolouration |
2 |
2 |
2 |
2 |
2 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
||
5 / M |
2000 |
Focal crusts |
. |
. |
. |
. |
. |
1 |
1 |
. |
. |
. |
. |
. |
. |
. |
Brown discolouration |
2 |
2 |
2 |
2 |
2 |
1 |
1 |
1 |
1 |
1 |
. |
. |
. |
. |
||
6 / M |
2000 |
Brown discolouration |
2 |
2 |
2 |
2 |
2 |
1 |
1 |
. |
. |
. |
. |
. |
. |
. |
7 / F |
2000 |
Brown discolouration |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
. |
8 / F |
2000 |
Brown discolouration |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
9 / F |
2000 |
Focal crusts |
. |
. |
. |
. |
. |
1 |
1 |
. |
. |
. |
. |
. |
. |
. |
Brown discolouration |
2 |
2 |
2 |
2 |
2 |
2 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
||
10 / F |
2000 |
Brown discolouration |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
. |
. |
. |
. |
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The median lethal dose (LD50) of the test item after single dermal administration to rats of both sexes, observed over a period of 14 days, is greater than 2000 mg/kg body weight. The study is considered to be adequate and reliable.
- Executive summary:
Acute dermal toxicity was assessed in a valid GLP study according to OECD TG 402.
5 male and 5 female RccHan:WIST (SPF) rats were treated with the test item at a dose level of 2000 mg/kg by dermal application. The test item was applied by weight as supplied by the Sponsor. For better dermal contact, the test item was moistened with purified water before application. All animals were examined for clinical signs before treatment, once within the first 30 minutes and approximately 1, 2, 3 and 5 hours after treatment on test day 1 and once daily during test days 2 to 15. Local dermal signs were assessed once daily from test day 2 to 15. Mortality was assessed before treatment, once within the first 30 minutes and approximately 1, 2, 3 and 5 hours after treatment on test day 1 (with the clinical signs) and twice daily during test days 2 to15. Body weights were recorded on test day 1 (prior to administration) and on test days 8 and 15. All animals were examined macroscopically after being sacrificed at the end of the study.
No deaths occurred during the course of the study and no clinical signs were observed. Four males and one female showed focal crusts after test item application lasting for a minimum of 2 and a maximum of 13 days. Brown discoloration of the skin was observed in all animals for a minimum of 7 days after test item application and lasted up to the end of the observation period in 3 males and 3 females. One female slightly lost body weight in the second week after treatment (2%). The body weights of the other animals were within the range commonly recorded for this strain and age. No macroscopic findings were recorded at necropsy.
The median lethal dose of the test item after single dermal administration to rats of both sexes, observed over a period of 14 days, is greater than 2000 mg/kg body weight.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.