Thiamine hydrochloride

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19 October 1992 to 17 November 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1984

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Composite samples (about 150 mL in duplicate) of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate centre of the test vessels.
- They were taken immediately before the exposure and after 96 hours exposure and kept at -18 to -22 °C until analysis.

Vehicle:

no

Details on test solutions:

- 10000 mg test material was mixed and made up to 500 mL with water.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow Trout
- Length at study initiation (mean): 51 mm (43-56 mm) based on 10 fish
- Weight at study initiation (mean): 1.36 g (0.90—1.65 g) based on 10 fish

ACCLIMATION
- Acclimation period: 14 days
- No food 24 hours prior to exposure

FEEDING DURING TEST: No

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

172 mg CaCO3/L

Test temperature:

14 ± 1 °C

pH:

7.7 - 8.2

Dissolved oxygen:

95 - 103 % O2

Nominal and measured concentrations:

Nominal: 10, 18, 32, 58 and 100 mg/L.
Measured (0 hours): 10.1, 18.5, 30.7, 55.2 and 94.7 mg/L
Measured (96 hours): 10.0, 18.1, 30.5, 54.8 and 94.3 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria of 20 litres filled with 15 litres. (36/22/25 cm)
- Aeration: Gentle aeration during exposure.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 0.45 g/L

TEST MEDIUM / WATER PARAMETERS
- Dechlorinated tap water (carbon filter)
- Intervals of water quality measurement: The oxygen content, pH and temperature were measured at 0, 24, 48, 72 and 96 hours.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: Fluorescent light, 16 hours daily


EFFECT PARAMETERS MEASURED:
- Mortalities were recorded after 24, 48, 72 and 96 hours exposure.
- The fish were examined daily for the following symptoms: swimming behaviour, loss of equilibrium, respiratory function, exophthalmos and pigmentation.

TEST CONCENTRATIONS
- 10, 18, 32, 58 and 100 mg/L.

Reference substance (positive control):

not specified

Key result

Duration:

95 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- No mortalities occurred during the study.
- No symptoms (swimming behaviour, loss of equilibrium, respiratory function, exophthalmos or pigmentation) were observed in any group at any time during the study.

Reported statistics and error estimates:

No statistical analysis was performed.

Validity criteria fulfilled:

not specified

Conclusions:

Under the conditions of this study the 96 hour EC50 was >100 mg/L, the 96 hour NOEC and LC0 were 100 mg/L and the 96 hour LC100 was >100 mg/L. The 24, 48 and 72 hour EC50 values were all >100 mg/L.

Executive summary:

The acute toxicity of the test material to fish was investigated in accordance with the standardised guideline OECD 203, under GLP conditions.

Rainbow Trout (Oncorhynchus mykiss) were exposed to the test material for 96 hours at the nominal concentrations of 10, 18, 32, 58 and 100 mg/L. Mortalities were recorded after 24, 48, 72 and 96 hours exposure. The fish were examined daily.

The test material concentrations were verified analytically using UV-Spectroscopy, the measured concentrations at 0 hours were 95 – 103 % of the nominal concentrations and measured concentrations at 96 hours were 94 – 101 % of the nominal concentrations.

No mortalities occurred during the study and no symptoms (swimming behaviour, loss of equilibrium, respiratory function, exophthalmos or pigmentation) were observed in any group at any time during the study.

Under the conditions of this study the 96 hour EC50 was >100 mg/L, the 96 hour NOEC and LC0 were 100 mg/L and the 96 hour LC100 was >100 mg/L. The 24, 48 and 72 hour EC50 values were all >100 mg/L.

Description of key information

Under the conditions of this study the 96 hour EC50 was >100 mg/L, the 96 hour NOEC and LC0 were 100 mg/L and the 96 hour LC100 was >100 mg/L. The 24, 48 and 72 hour EC50 values were all >100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

The acute toxicity of the test material to fish was investigated in accordance with the standardised guideline OECD 203, under GLP conditions. The study was awarded a reliability score of 1 in accordance with the criteria set forth by Klimisch et al. (1997).

Rainbow Trout (Oncorhynchus mykiss) were exposed to the test material for 96 hours at the nominal concentrations of 10, 18, 32, 58 and 100 mg/L. Mortalities were recorded after 24, 48, 72 and 96 hours exposure. The fish were examined daily.

The test material concentrations were verified analytically using UV-Spectroscopy, the measured concentrations at 0 hours were 95 – 103 % of the nominal concentrations and measured concentrations at 96 hours were 94 – 101 % of the nominal concentrations.

No mortalities occurred during the study and no symptoms (swimming behaviour, loss of equilibrium, respiratory function, exophthalmos or pigmentation) were observed in any group at any time during the study.

Under the conditions of this study the 96 hour EC50 was >100 mg/L, the 96 hour NOEC and LC0 were 100 mg/L and the 96 hour LC100 was >100 mg/L. The 24, 48 and 72 hour EC50 values were all >100 mg/L.