Trichlorotrifluoroethane

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Data source

Reference

Reference Type:

publication

Title:

Pouvoir irritant des principaux solvants chlorés aliphatiques sur la peau et les muqueuses oculaires du lapin

Author:

Duprat P, Delsaut L, Gradiski D

Year:

1976

Bibliographic source:

Eur J Toxicol. 1976, 9(3) 171-7

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The test item 1,1,2-trichloro-1,2,2-trifluoroethane was applied into the eye of six New-Zealand rabbits. After an unknown exposure time, the severity of irritation/corrosion was quantified. Classification was based on the method of Kay and Calandra. Histological examination of the treatment site was performed.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,1,2-trichlorotrifluoroethane

EC Number:

200-936-1

EC Name:

1,1,2-trichlorotrifluoroethane

Cas Number:

76-13-1

IUPAC Name:

1,1,2-trichloro-1,2,2-trifluoroethane

Details on test material:

- Name of test material (as cited in study report): "trichloro trifluoro-1,1,2-ethane", "trichloro 1,1,2-trifluoro-ethane"
- Molecular formula: C2Cl3F3 (identical to submission substance)
- Molecular weight: 187.376 g/mol (identical to submission substance)
- Smiles notation: ClC(F)(F)C(Cl)(Cl)F (different from submission substance)
- InChl: InChI=1S/C2Cl3F3/c3-1(4,6)2(5,7)8 (different from submission substance)
- Structural formula: see attachment CAS_76-13-1_Structure.png (under "Illustration (picture/graph)")
- Physical state: liquid
- Analytical purity: > 99%
- Supplier: "Flucka"

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.4-3.0 kg
- Sex: female

Test system

Vehicle:

not specified

Controls:

no

Amount / concentration applied:

no data

Duration of treatment / exposure:

no data

Observation period (in vivo):

no data

Number of animals or in vitro replicates:

6 animals per test item were used

Details on study design:

SCORING SYSTEM: According to the method described by Kay and Calandra (Kay JH, Calandra JC, Interpretation of eye irritation tests, J Soc Cosmetic Chem. 1962, 13: 281-289)

TOOL USED TO ASSESS SCORE: microscope / fluorescein

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

24/48/72 h

Score:

0.2

Max. score:

110

Irritant / corrosive response data:

The test item 1,1,2-trichloro-1,2,2-trifluoroethane showed practically no effect on the eyeball. Histopathologic examination showed only a slight augmentation of the corneal epithelium.

Any other information on results incl. tables

The test item 1,1,2-trichloro-1,2,2-trifluoroethane showed practically no effect on the eyeball. Histopathologic examination showed only a slight augmentation of the corneal epithelium.

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: other: Kay JH, Calandra JC, J Soc Cosmetic Chem. 1962, 13: 281-9

Conclusions:

The eye irritation potential of the test substance 1,1,2-trichloro-1,2,2-trifluoroethane was assessed in an in vivo test on six rabbits. Results revealed that the test item is not irritating showing a primary dermal irritation score of 0.2 (with 110 being the maximum value). The only alteration observed was a slight augmentation of the corneal epithelium. Thus the test item is not irritating to the eyes.

The eye irritation potential of the target chemical 1,1,1-trichloro-2,2,2-trifluoroethane is determined by read-across from an in vivo test on six rabbits with the source chemical 1,1,2-trichloro-1,2,2-trifluoroethane. The analogue approach is justified in Section 13 (Assessment Reports_Read-Across, in attachment CAS_354-58-5_Read-Across.pdf). As a consequence 1,1,1-trichloro-2,2,2-trifluoroethane is not considered as irritating to the eyes.

Executive summary:

The eye irritation potential of the test substance 1,1,2-trichloro-1,2,2-trifluoroethane was assessed in an in vivo test on six rabbits. Results revealed that the test item is not irritating showing a primary dermal irritation score of 0.2 (with 110 being the maximum value). The only alteration observed was a slight augmentation of the corneal epithelium. Thus the test item is not irritating to rabbit eyes.

The eye irritation potential of the target chemical 1,1,1-trichloro-2,2,2-trifluoroethane is determined by read-across from an in vivo test on six rabbits with the source chemical 1,1,2-trichloro-1,2,2-trifluoroethane. The analogue approach is justified in Section 13 (Assessment Reports_Read-Across, in attachment CAS_354-58-5_Read-Across.pdf). As a consequence 1,1,1-trichloro-2,2,2-trifluoroethane is not considered as irritating to rabbit eyes.