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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report date:
1999

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Oxirane, 2-methyl-, oligomeric reaction products with oxirane, ether with 2-ethyl-2-(hydroxymethyl)-1,3-propanediol (3:1)
EC Number:
610-877-7
Cas Number:
52624-57-4
Molecular formula:
C6 H14 O3 . 3 (C3 H6 O . C2 H4 O)x
IUPAC Name:
Oxirane, 2-methyl-, oligomeric reaction products with oxirane, ether with 2-ethyl-2-(hydroxymethyl)-1,3-propanediol (3:1)
Details on test material:
- Name of test material (as cited in study report): Triol 9053
- Physical state: colourless-yellowish liquid
- Analytical purity: not reported
- Batch No.: Kreisprobe Tank 16
- Storage condition of test material: at room temperature

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: Activated sludge from Iaboratory wastewater plant treating municipal sewage.
- Concentration of sludge: 30 mg/L
Duration of test (contact time):
42 d
Initial test substance concentrationopen allclose all
Initial conc.:
20 mg/L
Based on:
DOC
Initial conc.:
35 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Composition of medium: 1000 ml per vessel containing 65 ml test substance, 5 ml inoculum, 13 ml inorganic medium and 917 ml deionized water
- pH: 7.4

SAMPLING
- Sampling frequency: after 0, 1, 3, 5, 7, 10, 14, 17, 21, 24, 27, 28, 31, 35, 41, 42 days

CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 replicates
- Abiotic sterile control: 1replicate
- Inhibition control: 1 replicate
- Reference substance: 1 replicate
- Adsorption control: 1 replicate

Reference substance
Reference substance:
aniline

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (DOC removal)
Value:
60 - 70
Sampling time:
28 d
Parameter:
% degradation (DOC removal)
Value:
70 - 80
Sampling time:
42 d
Details on results:
Degradation kinetic:
3% after Day 1
14% after Day 3
50% after Day 14
65% after Day 28
77% after Day 41

BOD5 / COD results

Results with reference substance:
Degredation kinetic:
3% after Day 1
86% after Day 3

Applicant's summary and conclusion

Interpretation of results:
other: biodegradable