2,4-dichloro-1-(trichloromethyl)benzene

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1972

Report date:

1972

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Principles of method if other than guideline:

Main deviations from guideline:
2 instead of 5 animals per dose;
observation period was only 7 days instead of at least 14 days;
occlusive dressig instead of semiocclusive;
no information about per cent surface area covered

GLP compliance:

no

Test type:

other: range-finding test

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,4-Dichlorobenzotrichloride

IUPAC Name:

2,4-Dichlorobenzotrichloride

Constituent 2

Reference substance name:

2,4-dichloro-1-(trichloromethyl)benzene

EC Number:

235-868-1

EC Name:

2,4-dichloro-1-(trichloromethyl)benzene

Cas Number:

13014-18-1

Molecular formula:

C7H3Cl5

IUPAC Name:

2,4-dichloro-1-(trichloromethyl)benzene

Test material form:

solid: crystalline

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

not specified

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

SKIN
the test item was applied to the clipped intact (half the animals) or abraded (half the animals) abdominal skin

COVERAGE
- Type of wrap if used: binder of rubber dam

REMOVAL OF TEST SUBSTANCE
- residue material wiped off

Duration of exposure:

24 hours

Doses:

0.316, 1.0, 3.16, 10 g/kg bw

No. of animals per sex per dose:

2 animals per dose

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: gross necropsies were performed on the animals which died during the study

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

approximate LD50

Effect level:

> 10 000 mg/kg bw

Based on:

test mat.

Mortality:

One animal died in the test group receiving 3.16 g/kg

Clinical signs:

other: One rabbit at the 3.16 g/kg bw level was depressed through Day 2 and exhibited anorexia on Days 6 and 7; in the other animal at this level, death was preceded by depression, ataxia and anorexia. Both rabbits at the highest level exhibited signs through Da

Gross pathology:

None at the three lower levels. Both high level animals had spotted discoloration of the liver.

Other findings:

Slight to moderate erythema and edema were oserved. At higher concentration levels erythema were severe. No irritation scores reported. Blanching, desquamation, and occasional coriaceous appearance and necrosis.

Applicant's summary and conclusion

Conclusions:

Under the conditions of the present study the LD50 value for the test item was found to be > 10000 mg/kg bw.