Methyl 2-chloropropionate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Report states study in accordance with EEC Methodology however no specific method was cited.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Materials and methods

Test guideline

Guideline:

other: Report states study in accordance with EEC Methodology however no specific method was cited.

GLP compliance:

not specified

Test type:

other: single dermal application

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-Chloropropionic acid methyl ester

IUPAC Name:

2-Chloropropionic acid methyl ester

Details on test material:

- Name of test material (as cited in study report): MeACPA/2-Chloropropionic acid methyl ester
- Physical state: Liquid
- Analytical purity: 99.4%

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Type of coverage:

not specified

Vehicle:

not specified

Duration of exposure:

Not specified

Doses:

2000mg/kg bw

No. of animals per sex per dose:

2 animals per sex per dose

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

>= 2 000 mg/kg bw

Based on:

not specified

Any other information on results incl. tables

A summary of the dermal reactions observed is presented in Table 1.

Table1.

Dose (mg/kg bw)	Sex	Animal Number	E = Erythema O = Oedema	Day
				2	3 to 8
2000	Male	1	E	0	0
			O	0	0
		2	E	0	0
			O	0	0
	Female	3	E	0	0
			O	0	0
		4	E	1	0
			O	1	0

There was no evidence of systemic toxicity in any animal throughout the study. Very slight dermal irritation was observed in one female, resolving by day 3. Body weight gains were considered normal. No abnormalities were observed at macroscopic examination.

Applicant's summary and conclusion

Interpretation of results:

not classified

Conclusions:

Based on the results of the study the LD50 of MeACPA was shown to be greater than 2000 mg/kg bw.

Executive summary:

The dermal effect of Methyl-2-chloropropionate on rats was evaluated by a study in accordance with EEC methodology. Two male and two female Sprague-Dawley rats were dosed at 2000 mg/kg/bw. The animals were observed and clinical signs recorded for a period of 8 days after which the animals were sacrificed.

No deaths occurred. There was no evidence of systemic toxicity in any animal. Very slight dermal irritation was observed in one female however this was no longer evident by day 3. Body weight gains were considered normal for this type of study. No abnormalities were evident at the macroscopic examination.

In conclusion the acute lethal dermal dose for Methyl-2-chloropropionate was shown to be > 2000 mg/kg/bw.

The toxicity end point for the dermal route of exposure is above the 2000 mg/kg/bw threshold for classification under the CLP regulation; therefore no classification relating to dermal toxicity is required.