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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions: Incomplete documentation, TA 102 or E.coli WP2 were not tested

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: see Principles of method if other than guideline
Principles of method if other than guideline:
Ames BN et al. (1975). Mutat. Res. 31, 347-364
GLP compliance:
no
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Reference substance name:
cis-3,3,5-trimethylcyclohexanol
IUPAC Name:
cis-3,3,5-trimethylcyclohexanol
Details on test material:
cis-3,3,5-trimethylcyclohexanol of Hüls AG, ID 536/880202, commercial quality, purity not reported

Method

Species / strain
Species / strain / cell type:
other: Salmonella typhimurium TA 1535, TA 1537, TA 1538, TA 98, TA 100
Metabolic activation:
with and without
Test concentrations with justification for top dose:
10; 50; 250; 1000; 2000; 5000 µg/plate
Vehicle / solvent:
Dimethyl sulfoxide (CAS: 67-68-5)
Details on test system and experimental conditions:
Type: Ames test

Results and discussion

Test results
Species / strain:
other: Salmonella typhimurium TA 1535, TA 1537, TA 1538, TA 98, TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
other: >= 1000 µg/plate
Vehicle controls validity:
valid
Untreated negative controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: other: Salmonella typhimurium TA 1535, TA 1537, TA 1538, TA 98, TA 100
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

GENOTOXIC EFFECTS: 
- With metabolic activation: None
- Without metabolic activation: None
  The positive controls were functional.
PRECIPITATION CONCENTRATION: No precipitation was observed.
CYTOTOXIC CONCENTRATION: 
  TA 98: 5000 µg/plate (+/- S9)
  TA 100: 5000 µg/plate (+/- S9)
  TA 1535: 5000 µg/plate (+ S9), 250/1000 µg/plate (- S9)
  TA 1537: 5000 µg/plate (+ S9), 1000 µg/plate (- S9)
  TA 1538: 5000 µg/plate (+/- S9)

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative +/- S9

The test substance was found not to be genotoxic in an Ames test with and without metabolic activation
Executive summary:

The test substance was found not to be genotoxic in an Ames test with and without metabolic activation.