D-cellobiose

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 13 - ongoing

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

April 13, 2004

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

LOT No.: L1021174
Appearance: white, dry powder
Expiry date: 28 October 2023
Storage condition: at room temperature, protected from light
Safety precautions: according to MSDS
Purity: 99.54 %

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L

Vehicle:

yes

Remarks:

dilution water (ISO Medium)

Details on test solutions:

The test solution used in the test was prepared by mechanical dispersion without using of any solubilising agent, as the substance is well water soluble.
The test solution was prepared by dissolving an amount of 0.0304 g test item in 304 mL dilution water (ISO Medium) to obtain a nominal concentration of 100 mg/L. After the formulation procedure the test animals were immediately introduced into test solution. Untreated control ran in parallel.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain/clone: Daphnia magna (Straus)
- Age at study initiation (mean and range, SD): less than 24h
- Method of breeding: -
- Source: TOXI-COOP ZRT
- Feeding during test: NO

ACCLIMATION
- Acclimation period: Test animals were bred under similar (or the same) conditions as that used during the exposure period (holding water, temperature, background colour etc.), therefore additional acclimatisation before the test was not necessary. Brood daphnids were maintained in dilution water at the test temperature for at least 48 hours prior to the start of the test.
- Type and amount of food: algal suspension of Raphidocelis subcapitata

:

Test type:

static

Water media type:

freshwater

Remarks:

Reconstituted water (ISO Medium, according to OECD 202)

Limit test:

yes

Total exposure duration:

48 h

Hardness:

approximate theoretical total hardness of 249 mg/L (as CaCO3), calculated based on the concentration of calcium and magnesium ions in ISO medium

Test temperature:

19.8 – 20.0°C

pH:

7.24 – 7.65

Dissolved oxygen:

of 8.44 – 8.78 mg/L

Nominal and measured concentrations:

nominal: 100 mg/L
measured: 88.6 – 113.0 mg/L (i.e. within ± 20% of the nominal)

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

- Behavioural abnormalities: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Immobilisation of control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no

Results with reference substance (positive control):

- Results with reference substance valid? YES
- Relevant effect levels: immobilization
- Limit test: no
- ECx: 24h EC50: 1.06 mg/L (95 % confidence limits: 1.02 – 1.10 mg/L)

Validity criteria fulfilled:

yes

Conclusions:

In a GLP-compliant study according to OECD TG 202 with Daphnia magna, cellobiose did not induce any effects at the limit concentration of 100 mg/L.

Executive summary:

The purpose of this study was to evaluate the influence of the test item on the mobility respectively survival of Daphnia magna.

Young Daphnia were exposed to the test item in aqueous test media for 48 hours under defined conditions. The number of Daphnia no longer capable of swimming at the end of the test or being dead was recorded.

In a preliminary test, the test item revealed no toxic effect on daphnids up to a concentration of 100 mg/L (limit concentration). Therefore, a limit test was performed using only this concentration level and a control in order to demonstrate that the test item is not toxic to Daphnia magna up to at least the concentration of 100 mg/L, in line with the OECD 202 guideline.

The test was run according to international test guidelines using the recommended test species Daphnia magna.

Description of key information

In a GLP-compliant study according to OECD TG 202 with Daphnia magna, cellobiose did not induce any effects at the limit concentration of 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L