Potassium carbamoylcarbamate

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD 301D

Principles of method if other than guideline:

The purpose of this study was to evaluate the aerobic biodegradability of potassium allophonate
in water containing mineral salts and the secondary effluent from a wastewater treatment plant as
the source of microbial inoculum.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Potassium carbamoylcarbamate

EC Number:

247-728-7

EC Name:

Potassium carbamoylcarbamate

Cas Number:

26479-35-6

Molecular formula:

C2H3KN2O3

IUPAC Name:

potassium N-carbamoylcarbamate

Details on test material:

- Name of test material (as cited in study report): Potassium Allophonoate
- Lot/batch No.: K10-272

Sampling and analysis

Analytical monitoring:

not required

Details on sampling:

The percent degradation for the test substance treatment on Days 7, 14, 21, and 28

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Bacterial plate counts were performed on the filtered secondary effluent inoculum at initiation and
one of the duplicate BOD bottles for each treatment at Day 28. A 1 mL sample of buffered water
was plated directly to check for sterility. An agar blank was prepared by adding only agar to a Petri
dish. Duplicate 1-mL aliquots of each dilution were directly analyzed by plate counting methods
patterned after methods described in Standard Methods for the Examination of Water and
Wastewater (3). The bacterial growth medium was Plate Count Agar (Difco Laboratories). The
plates were incubated at 26 + 2°C for six days before counting the number of colonies on plates
with greater than 30, but fewer than 300 colonies. The number of colonies at the dilution coming
closest to 300 colonies was used to calculate colony forming units (CFU)/mL for each BOD bottle
sampled.

Study design

Test type:

static

Total exposure duration:

28 d

Results and discussion

Effect concentrations

Key result

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: viability

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The results
from bacterial plate count analyses indicated that the test substance was not toxic to the
secondary effluent inoculum at a concentration of 5.0 mg/L total product.