Dimethylaminopropylmethacrylamide, dodecyl bromide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22/03/2016 - 07/10/2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

N-(3-methacrylamidopropyl)-N,N-dimethyldodecan-1-aminium bromide

Cas Number:

129684-50-0

Molecular formula:

C21H43N2O.Br

IUPAC Name:

N-(3-methacrylamidopropyl)-N,N-dimethyldodecan-1-aminium bromide

Test material form:

liquid: viscous

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The study was performed on the freshwater crustacean Daphnia magna Straus. A strain of this species was supplied by MicroBioTests Inc., Belgium in December 2011 (batch number: DM290911).
Since that time, the clone has been bred in the laboratories of the Test Facility under conditions identical to those of the test, with regard to temperature, light and water quality (see below), so that no acclimation was needed prior to testing.
It was daily fed with a fixed amount of the laboratory cultured green algae Pseudokirchneriella subcapitata (formerly Selenastrum capricornutum) and with a suspension of the yeast Saccharomyces cerevisiae.
At the test start ,the organisms used in the test were less than 24 hours old and were not first brood progeny."

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

140 - 250 mg/L as CaCO3

Test temperature:

20.0 - 20.2°C

pH:

7.54 - 7.97

Dissolved oxygen:

5.68 - 6.84 mg/L

Nominal and measured concentrations:

Negative control (reconstituted water without test item) and 0.6, 1.3, 2.5, 5.0 and 10.0 mg test item/L (Nominal concentrations)

Details on test conditions:

The test was performed in glass beakers filled with 40 mL of test medium. The test vessels were covered with glass plates to reduce the loss of water and to avoid dust entry into test solutions.
The test vessels were labelled with the internal study number and all necessary additional information to ensure unmistakable identification.
For the test concentrations and for the negative control, group of 20 daphnids were used divided into four replicates of five daphnids each. The daphnids were randomly distributed to the test vessels at test initiation. The daphnids loading rate was about 8 mL per one daphnid (5 daphnids for 40 mL, according to guideline OECD 202, 2004).

Temperature:
The incubation temperature was continuously monitored during the course of the study by a PT100 probe installed in the environmental test chamber. It was in the range 20.0 – 20.2°C with a mean value of 20.0 °C and a standard deviation of 0.04 °C. The temperature range provided by OECD guideline is 20 ± 2°C. The temperature values during the test were within the provided range and did not vary more than 1°C during the test period.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

IC50

Effect conc.:

2.3 mg/L

95% CI:

1.9 - 2.7

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, the IC50/Daphnia magna at 48 hours was 2.3 mg/L.