[No public or meaningful name is available]

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19 January 2022

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2022

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Enzymatically produced steviol glycosides

IUPAC Name:

Enzymatically produced steviol glycosides

Test material form:

solid: particulate/powder

Test solutions

Vehicle:

yes

Remarks:

OECD alga medium

Details on test solutions:

OECD alga growth medium was prepared by adding appropriate volume of the stock solutions 1 - 4 (10 mL of stock solution 1, 1 mL of stock solution 2, 1 mL of stock solution 3, 1 mL of stock solution 4) to 500 mL of sterilized deionized water and made up to 1000 mL. Sufficient time was given for equilibrating the medium with the atmospheric CO2 and pH was set to 8.1.

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Post exposure observation period:

no

Test conditions

Test temperature:

During the test period all the culture flasks were maintained at the test medium temperature of 22.3º to 22.9ºC during dose range finding study and temperature of 22.3º to 22.8ºC during limit test.

pH:

The pH of the test nominal loading rates was ranged from 7.96 to 8.21 at the beginning (0 h) and 8.00 to 8.22 at the end of the test (72 h) during dose range finding study.
The pH of the test nominal loading rates was ranged from 7.67 to 7.83 at the beginning (0 h) and 7.61 to 7.81 at the end of the test (72 h) during limit test.

Reference substance (positive control):

yes

Remarks:

3, 5 Dichlorophenol 97%: NOEC=0.10mg/L; LOEC=0.23mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EL10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOELR

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 5

Duration:

72 h

Dose descriptor:

LOELR

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 72 hours EL10, EL20, EL50 values based on the growth rate and yield in terms of nominal loading rate are greater than 100 mg/L.
NOELR and LOELR based on the growth rate and yield were calculated in terms of test nominal loading rates are 100 mg/L and greater than 100 mg/L respectively.