Sodium 2-hydroxypropane-2-sulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 January 2001 until 25 January 2001

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test substance was stirred in M4 medium for about 10 minutes at 20 +- 2°C.
The nominal concentration of the stock solution was 100 mg/l.
By diluting this stock solution with M4 medium the following nominal concentrations (mg/l) were prepared:
control, 100, 50, 25 and 12.5

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Straus 1820
- Age at study initiation (mean and range, SD): 2 -24 hours
- Source: Laboratory Experimental Toxicology and Ecology of BASF AG in Ludwigshafen
- Age of parental stock (mean and range, SD): 2-4 weeks

Test type:

static

Water media type:

freshwater

Remarks:

standard M4 medium

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.2 - 3.2 mmol/l

Test temperature:

18 - 22°C

pH:

7.5 -8.5

Dissolved oxygen:

M4 medium is aerated for approximately 24 hours until saturation with oxygen is reached

Conductivity:

550 - 650 µS/cm

Nominal and measured concentrations:

Nominal concentrations: control, 100, 50, 25 and 12.5 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom (nominal volume 20 ml)
- Volume of solution: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

- Light intensity: 2 - 7 µE/(m²s)
wave length: 400 - 700 nm

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

Based on the nominal concentrations the EC50 is greater than 100 mg/L.

Executive summary:

The study was performed to determine the toxic effect on Daphnia magna in this 48 hour acute toxicity test.

Based on the nominal concentrations the EC50 is greater than 100 mg/L. No significant effect was observed in this study.

Description of key information

In a 48-hour acute test (BASF, 00/0802/50/1) with Daphnia magna, the EC50 for this substance was determined at 100 mg/L, based on analytically measured concentrations.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L