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Diss Factsheets

Ecotoxicological information

Toxicity to terrestrial arthropods

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to bees: acute contact
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
other: Subdivision L, Hazard Evaluation, Nontarget Insects. Environmental Protection Agency, EPA 540/9-82-019
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: United Kingdom Pesticides Safety Precautions Scheme. Laboratory testing for toxicity to honeybees. London: Ministry of Agriculture, Fisheries and Food,
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: United Kingdom Pesticides Safety Precautions Scheme. Testing for toxicity to foraging bees under field conditions. London: Ministry of Agriculture, Fisheries and Food
Deviations:
no
GLP compliance:
no
Application method:
contact
Specific details on test material used for the study:
Haloxyfop-R methyl ester
Purity: Not reported
Analytical monitoring:
no
Vehicle:
yes
Remarks:
Deionized water
Test organisms (species):
Apis mellifera
Animal group:
Hymenoptera (honeybees)
Details on test organisms:
TEST ORGANISM
- Common name: Honey bee
- Age: Young worker bees
- Disease free: Yes

ACCLIMATION
- Acclimation period: 2 days
Study type:
laboratory study
Limit test:
no
Total exposure duration:
48 h
Test temperature:
23.0-24.0˚C
Humidity:
68%
Photoperiod and lighting:
darkness
Details on test conditions:
TEST SYSTEM
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 3
- No. of replicates per control: 3
Nominal and measured concentrations:
0.1, 1.0, 10.0, 100 µg/bee
Key result
Duration:
48 h
Dose descriptor:
LD50
Effect conc.:
> 100 µg per animal
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: highest dose tested
Conclusions:
48 h LD80 (honey bee): >100 µg/bee (highest dose tested)
Executive summary:

This study was carried out to determine the acute contact toxicity to honeybees (Apis mellifera) of the test substance. Worker bees in four groups of thirty were topically dosed with the following doses: 0.1, 1.0, 10.0, 100 µg/bee. After 48 hours exposition, no significant toxic effects were observed in all tested doses. Therefore, the median lethal dose of the test substance (48-h LD50), under the test conditions and based on nominal dose, was considered greater than 100 µg/bee.

Description of key information

48 h LD50 (honey bee): 100 µg/bee (highest dose tested); USEPA. Pesticide Assessment Guidelines. Subdivision L, Hazard Evaluation, EPA 540/9-82-019; Reliability = 2

Key value for chemical safety assessment

Additional information