Phosphoric acid, C8-16-alkyl esters, reaction products with (Z)-N-9-octadecenyl-1,3-propanediamine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Phosphoric acid, C8-16-alkyl esters, reaction products with (Z)-N-9-octadecenyl-1,3-propanediamine

EC Number:

291-909-3

EC Name:

Phosphoric acid, C8-16-alkyl esters, reaction products with (Z)-N-9-octadecenyl-1,3-propanediamine

Cas Number:

90506-47-1

Molecular formula:

complex substance

IUPAC Name:

Phosphoric acid, C8-16-alkyl esters, reaction products with (Z)-N-9-octadecenyl-1,3-propanediamine

Test material form:

liquid: viscous

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and batch No.of test material: ARCOT 3135 from PCAS, Batch N° 80555C079
- production date: May 2015
- date of receipt: 08 June 2015
- Expiration date of the batch: May 2016
- Purity test date: 28 May 2015

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature (20 ±5°C), in proper conditions in a closed vessel

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia
- Species: Daphnia magnia
- Variety: Straus
- Strain: Berlin
- Justification for species other than prescribed by test guideline: as recommended in the OECD guidline
- Source: Umweltbundesamt Berlin
- Selection of daphina: the newborn Daphnia, aged between 0 and 23 hours, were sieved from the medium and immediately placed into a 250 mL-beaker containing dilution water. After a settling-in period of 30 minutes, animals which showed no apparent damage were used for the test.
- Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

Observation times : 24 and 48h.

Test conditions

Hardness:

2.502 mmol/L ; 250 mg of CaCO3/L

Test temperature:

19.5 - 20.8°C

pH:

adjusted to 7.6 (varying between 7.7 and 7.8)

Dissolved oxygen:

O2 concentration between 8.1 and 8.7 mg/L

Nominal and measured concentrations:

2.2 / 4.6 / 10 / 22 / 46 / 100 mg/L nominal concentration

Details on test conditions:

TEST SYSTEM
- Test vessel: Beakers, glass, nominal volume 50mL
- Type: open
- Aeration: yes
- Renewal rate of test solution: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per blank control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Medium: M4-Medium (recipe of ELENDT) (composition detailed in annex of the complete report).

OTHER TEST CONDITIONS
- Adjustment of pH and oxygen: The pH and the concentration of dissolved oxygen in the test vessels were measured at the beginning and at the end of the test.
- Photoperiod: 16/8 hours, using neon tubes
- Temperature: 20 ± 2°c

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

15 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

Calculation of results was performed with the help of validated software (Microsoft Excel@, ToxRat@ Professional). The estimation of the biological results was accomplished using the software ToxRat@ Professional, version 3.1 . The details of calculation are stated in the annex, page 21 of the final report attached to the Iuclid file. Biological results are stated using two significant digits.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 24h EC 50 is 22mg/L (95% confidence interval 18-27 mg/L).
The 48h EC 50 is 15mg/L (95% confidence interval 12-18 mg/L).
The 48h NOEC is 4.6mg/L.

Executive summary:

A study was conducted for the determination of short term toxicity of ARCOT 3135 against Daphnia magna STRAUS according to OECD 202 resp.EU C.2.

The study was performed using 6 concentrations ranging from 2.2 to 100 mg/L. nominal concentration. For each test concentration, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.

4 concentrations showed toxicity between 25 and 100% immobilisation. In the control, none of the animals were immobilised. The following results were determined for the test item ARCOT 3135 (species: Daphnia magna) :

The 24h EC 50 is 22 mg/L (95% confidence interval 18-27 mg/L).

The 48h EC 50 is 15 mg/L (95% confidence interval 12-18 mg/L).

The 48h NOEC is 4.6 mg/L.