Registration Dossier
Registration Dossier
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EC number: 269-503-2 | CAS number: 68259-00-7
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 March1994 to 22Aug1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Version / remarks:
- OECD Guideline for Testing of Chemicals No. 3028, Paris 1981.
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methyl 1-methyl-4-[(methylphenylhydrazono)methyl]pyridinium sulphate
- EC Number:
- 269-503-2
- EC Name:
- Methyl 1-methyl-4-[(methylphenylhydrazono)methyl]pyridinium sulphate
- Cas Number:
- 68259-00-7
- Molecular formula:
- C14H16N3.CH3O4S
- IUPAC Name:
- methyl 1-methyl-4-[(methylphenylhydrazono)methyl]pyridinium sulphate
- Test material form:
- solid: particulate/powder
- Details on test material:
- See study reports for batch and purity information
Constituent 1
- Specific details on test material used for the study:
- EN-Nr.: 3001
Purity: ca. 90%
Appearance: solid
Solubility: 40 g/I [in water]
Storage: room temperature
Expiration date : 09/98
Study design
- Oxygen conditions:
- aerobic
- Remarks:
- No deviations from guideline noted and aerobic conduct is stated for OECD 302B
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Sludge of a biological sewage treatment plant n(ARA-Reinach) collected on 19.04.94
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 25 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- Strain / Inoculation
Sludge of a biological sewage treatment plant (ARA-Reinach) collected on 19.04.94
Water I Nutrient
according to the specification of the EEC L 133 (pp. 99-105) Volume 31 resp. OECD Method 302B.
Temperature
22.0 ± 3°C (room temperature)
Test duration
28days
Lighting
Indirect daylight
Estimation Technique
TOC/DOC Analyzer I SHIMADZU TOC-5000
Test concentrations
25 mg/I DOC nominal
Reference substance
- Reference substance:
- diethylene glycol
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 21.6
- Sampling time:
- 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- The test substance was tested in concentration of 25 mg/I DOC. The bioelimination of the test substance after 28 days is: 21.6 % and the test item is therefore considered to be inherently biodegradable.
- Executive summary:
The test substance was tested in concentration of 25 mg/I DOC. The bioelimination of the test substance after 28 days is: 21.6 % and the test item is therefore considered to be inherently biodegradable.
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